Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Human Hela Cells; Lane3: Human A431 Cells.)
Rabbit anti-Human Calcyclin Polyclonal Antibody | anti-CACYBP antibody
Polyclonal Antibody to Calcyclin Binding Protein (CACYBP)
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Human Hela Cells; Lane3: Human A431 Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Human Hela Cells; Lane3: Human A431 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CACYBP, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CACYBP, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)
Immunohistochemistry (IHC)
(FITC staining on IHC-P; Samples: Human Hela Cells)
Immunohistochemistry (IHC)
(FITC staining on IHC-P; Samples: Human Hela Cells)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CACYBP: a nuclear/cytoplasmic protein involved in calcium-dependent ubiquitination and proteosomal degradation of target proteins. A possible molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). Interacts with proteins of the S100 family at physiological calcium concentrations. Component of some large E3 complexes and interacts directly with SIAH1, SIAH2 and SKP1A. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated. Two alternatively spliced isoforms have been described.
Protein type: Adaptor/scaffold; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 1q25.1
Cellular Component: beta-catenin destruction complex; cell body; cytosol; extracellular exosome; neuron projection; nuclear envelope lumen; nucleoplasm; SCF ubiquitin ligase complex
Molecular Function: protein binding; protein domain specific binding; protein homodimerization activity; ubiquitin protein ligase binding
Biological Process: aging; cardiac muscle cell differentiation; cellular response to calcium ion; positive regulation of DNA replication
Research Articles on CACYBP
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Product Notes
The CACYBP cacybp (Catalog #AAA2027246) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Calcyclin Binding Protein (CACYBP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calcyclin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CACYBP cacybp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calcyclin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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