Rabbit NECAP1 Polyclonal Antibody | anti-NECAP1 antibody

NECAP1 Antibody

Cat. Num. AAA9611930

• Gene Names: NECAP1; EIEE21
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NECAP1; Polyclonal Antibody; NECAP1 Antibody; anti-NECAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NECAP1 Antibody detects endogenous levels of NECAP1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 275

• Applicable Applications for anti-NECAP1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human NECAP1.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Plasma Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse brain tissue, using NECAP1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-NECAP1 antibody

Description: Involved in endocytosis.

Subunit Structure: Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity).

Similarity: The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself. The WXXF motif 1, which is preceded by an acidic residue and has a glycine in second position mediates specific interaction with AP-1. The WXXF motif 2, which is followed by the C-terminal carboxyl group negative charge, allows specific interaction with AP-2 (By similarity). Belongs to the NECAP family.

NCBI and Uniprot Product Information

• NCBI GI #: 31542528
• NCBI GeneID: 25977
• NCBI Accession #: NP_056324.2
• NCBI GenBank Nucleotide #: NP_056324.2
• UniProt Accession #: Q8NC96
• Molecular Weight: Observed: 40 kDa; Predicted: 30 kDa
• NCBI Official Full Name: adaptin ear-binding coat-associated protein 1
• NCBI Official Synonym Full Names: NECAP endocytosis associated 1
• NCBI Official Symbol: NECAP1
• NCBI Official Synonym Symbols: EIEE21
• NCBI Protein Information: adaptin ear-binding coat-associated protein 1
• UniProt Protein Name: Adaptin ear-binding coat-associated protein 1
• Protein Family: Adaptin ear-binding coat-associated protein
• UniProt Gene Name: NECAP1
• UniProt Synonym Gene Names: NECAP-1
• UniProt Entry Name: NECP1_HUMAN

NCBI Description

This gene encodes a protein containing two characteristic WXXF motifs. The encoded protein localizes to clathrin-coated vesicles, where it binds components of the adapter protein complexes and aids in endocytosis. Loss of function of this gene results in early infantile epileptic encephalopathy-21. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]