Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Fibroblast growth factor 4 (FGF4) Standard Curve.)
Mouse Fibroblast Growth Factor 4 (FGF4) ELISA Kit | FGF4 elisa kit
Mouse Fibroblast Growth Factor 4 (FGF4) ELISA Kit
Gene Names
Fgf4; KS3; hst; Fgfk; Hst1; kFGF; Fgf-4; hst-1; HBGF-4; Hstf-1
Reactivity
Mouse
Synonyms
Fibroblast Growth Factor 4 (FGF4); Mouse Fibroblast Growth Factor 4 (FGF4) ELISA Kit; FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF; fibroblast growth factor 4 splice isoform; heparin secretory transforming protein 1; human stomach cancer; transforming factor from FGF-related oncogene; kaposi; FGF4 elisa kit
Reactivity
Mouse
Specificity
Mouse Fibroblast growth factor 4 (FGF4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FGF4. No significant cross-reactivity or interference between Mouse FGF4 and analogues was observed.
Sequence Length
202
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Fibroblast growth factor 4 (FGF4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Fibroblast growth factor 4 (FGF4) Standard Curve.)
Related Product Information for FGF4 elisa kit
Description: This Mouse Fibroblast growth factor 4 (FGF4) ELISA Kit employs a two-site sandwich ELISA to quantitate FGF4 in samples. An antibody specific for FGF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Fibroblast growth factor 4 is a protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity.This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. Co-amplification of both genes was found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway.
Background: Fibroblast growth factor 4 is a protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity.This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. Co-amplification of both genes was found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
fibroblast growth factor 4
NCBI Official Synonym Full Names
fibroblast growth factor 4
NCBI Official Symbol
Fgf4
NCBI Official Synonym Symbols
KS3; hst; Fgfk; Hst1; kFGF; Fgf-4; hst-1; HBGF-4; Hstf-1
NCBI Protein Information
fibroblast growth factor 4
UniProt Protein Name
Fibroblast growth factor 4
Protein Family
UniProt Gene Name
Fgf4
UniProt Synonym Gene Names
Fgf-4; Kfgf; FGF-4; HBGF-4
Uniprot Description
Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Is essential for survival of the postimplantation mouse embryo. Required for normal limb and cardiac valve development during embryogenesis.
Research Articles on FGF4
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Product Notes
The Mouse FGF4 fgf4 (Catalog #AAA9718150) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718150 ELISA Kit recognizes Mouse FGF4. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor 4 (FGF4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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