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Western Blot (WB) (Western Blot analysis of NDRG2 on mouse brain (1) and mouse skeletal muscle (2) tissue lysate using anti-NDRG2 antibody at 1/1,000 dilution.)

Rabbit NDRG2 Polyclonal Antibody | anti-NDRG2 antibody

NDRG2 Polyclonal Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein affinity purified.
Synonyms
NDRG2; Polyclonal Antibody; NDRG2 Polyclonal Antibody; KIAA1248; SYLD; Antidepressant related protein ADRG123; Cytoplasmic protein Ndr1; DKFZp781G1938; FLJ25522; N myc downstream regulated gene 2; N myc downstream regulator 2; NDR1 related protein NDR2; NDRG 2; NDRG family member 2; NDRG1 related protein; Protein NDRG2; Protein Syld709613; Syld709613 protein; anti-NDRG2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NDRG2 polyclonal antibody detects endogenous levels of NDRG2 protein.
Purity/Purification
Protein affinity purified.
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-NDRG2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:100
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein within human NDRG2 1-150 aa.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of NDRG2 on mouse brain (1) and mouse skeletal muscle (2) tissue lysate using anti-NDRG2 antibody at 1/1,000 dilution.)

Western Blot (WB) (Western Blot analysis of NDRG2 on mouse brain (1) and mouse skeletal muscle (2) tissue lysate using anti-NDRG2 antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining NDRG2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining NDRG2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-NDRG2 antibody
The N-myc downstream regulated gene (NDRG) family is comprised of four members, namely NDRG1, NDRG2, NDRG3 and NDRG4, all of which share 57-65% homology. NDRG2 (NDRG family member 2), also known as SYLD, is a 371 amino acid protein that localizes to both the cytoplasm and the perinuclear region in neurons. Expressed at high levels in heart, brain, dendritic cells, salivary gland and skeletal muscle and at lower levels in liver and kidney, NDRG2 is thought to be involved in dendritic and neuronal cell differentiation and outgrowth. Additionally, NDRG2 expression is downregulated in a variety of carcinomas, including liver cancer, pancreatic cancer and meningioma, suggesting a possible role for NDRG2 in tumor suppression. NDRG2 is found in brain lesions of Alzheimer Disease (AD)-affected patients and is thought to be associated with the progression of AD. Five isoforms of NDRG2 exist due to alternative splicing events.

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
41kDa
Protein Family

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Product Notes

The NDRG2 (Catalog #AAA3013103) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDRG2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDRG2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1,000 ICC: 1:50-1:200 IHC: 1:50-1:100 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the NDRG2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDRG2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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