Western Blot (WB)
(N0555-04D is used in Western blot to detect NCOR1 in mouse thymus tissue lysate.)
Rabbit anti-Human, Mouse NCOR1 Polyclonal Antibody | anti-NCOR1 antibody
NCOR1, NT (Nuclear Receptor Co-repressor 1, N-CoR, KIAA1047) (AP)
ELISA: 1:1000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Western Blot (WB)
(N0555-04D is used in Western blot to detect NCOR1 in mouse thymus tissue lysate.)
Western Blot (WB)
(N0555-04D is used in Western blot to detect NCOR1 in mouse thymus tissue lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reactedMBS628541, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reactedMBS628541, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained withMBS628541.. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Ncor1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Ncor1 immunoreactivity is localized to nucleus significantly. )
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained withMBS628541.. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Ncor1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Ncor1 immunoreactivity is localized to nucleus significantly. )
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.[provided by RefSeq, Jun 2010]
Uniprot Description
N-CoR1: is a protein that regulates the activity of some transcription factors, including nuclear receptors, by promoting histone deacetylation and chromatin condensation, thereby altering the accessibility of various genes to the transcriptional machinery. Is known to play a role genetic programming including that for myogenesis. Is a component of a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid and the retinoic acid receptors in the absence of ligand. Interacts with the catalytic domain of HDAC9. Increased expression correlates with the loss of vitamin D responsiveness in aggressive androgen-independent prostate cancer cells. Its gene tends to be overexpressed in multiple-myeloma cell lines.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 17p11.2
Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; Sin3 complex; membrane; spindle microtubule; nuclear chromatin; histone deacetylase complex; cytoplasm; nucleus
Molecular Function: retinoid X receptor binding; protein binding; histone deacetylase regulator activity; sequence-specific DNA binding; histone deacetylase binding; thyroid hormone receptor binding; chromatin binding; transcription factor activity; transcription corepressor activity; nuclear hormone receptor binding
Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; negative regulation of JNK cascade; CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; transcription, DNA-dependent; regulation of multicellular organism growth; chromatin modification; thalamus development; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; spindle assembly; positive regulation of histone deacetylation; cholesterol homeostasis; transforming growth factor beta receptor signaling pathway; gene expression; circadian regulation of gene expression; negative regulation of phosphoinositide 3-kinase cascade
Research Articles on NCOR1
Similar Products
Product Notes
The NCOR1 ncor1 (Catalog #AAA6489495) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCOR1, NT (Nuclear Receptor Co-repressor 1, N-CoR, KIAA1047) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NCOR1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NCOR1 ncor1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCOR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
