Western Blot (WB)
(Western blot analysis of extracts from various samples, using NCKIPSD Antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: HepG2; )
Rabbit anti-Human, Mouse NCKIPSD Polyclonal Antibody | anti-NCKIPSD antibody
NCKIPSD Antibody
Gene Names
NCKIPSD; DIP; DIP1; ORF1; WISH; VIP54; AF3P21; SPIN90; WASLBP
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NCKIPSD; Polyclonal Antibody; NCKIPSD Antibody; 54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; 90 kDa SH3 protein interacting with Nck; AF3p21; dia interacting protein; Dia-interacting protein 1; Diaphanous protein-interacting protein; DIP; DIP-1; DIP1; NCK interacting protein with SH3 domain; NCK-interacting protein with SH3 domain; Nckipsd; ORF1; SH3 adapter protein SPIN90; SH3 protein interacting with Nck 90 kDa; SPIN90; SPN90_HUMAN; VIP54; WASLBP; WASP-interacting SH3-domain protein; WISH; wiskott-Aldrich syndrome protein-interacting protein; Wiskott-Aldrich syndrome protein-interacting protein; anti-NCKIPSD antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
NCKIPSD antibody detects endogenous levels of total NCKIPSD
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
722
Applicable Applications for anti-NCKIPSD antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human NCKIPSD
Subcellular Location
Nucleus. Colocalizes with DRF1 at membrane ruffles, and with Nck at Z-disks in mature cardiac myocytes.
Tissue Specificity
Highest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using NCKIPSD Antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: HepG2; )
Western Blot (WB)
(Western blot analysis of extracts from various samples, using NCKIPSD Antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: HepG2; )
Western Blot (WB)
(Western blot analysis NCKIPSD using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis NCKIPSD using Jurkat whole cell lysates)
Related Product Information for anti-NCKIPSD antibody
Function: Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.
Subunit Structure: Associates with the intermediate filaments, vimentin and desmin. Binds the first and third SH3 domains of NCK. Binds the proline-rich domains of N-WASP through its SH3 domain (By similarity). Similarly, binds diaphanous protein homolog 1 (DRF1). Binds the SH3 domains of GRB2 through its proline-rich domains. Interacts with Helicobacter pylori toxin vacA. Isoform 4 interacts with FHOD1. Interacts with FASLG. Interacts with TMIGD2.
Subunit Structure: Associates with the intermediate filaments, vimentin and desmin. Binds the first and third SH3 domains of NCK. Binds the proline-rich domains of N-WASP through its SH3 domain (By similarity). Similarly, binds diaphanous protein homolog 1 (DRF1). Binds the SH3 domains of GRB2 through its proline-rich domains. Interacts with Helicobacter pylori toxin vacA. Isoform 4 interacts with FHOD1. Interacts with FASLG. Interacts with TMIGD2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 79 kDa
Predicted: 79 kDa
Predicted: 79 kDa
NCBI Official Full Name
NCK-interacting protein with SH3 domain isoform 1
NCBI Official Synonym Full Names
NCK interacting protein with SH3 domain
NCBI Official Symbol
NCKIPSD
NCBI Official Synonym Symbols
DIP; DIP1; ORF1; WISH; VIP54; AF3P21; SPIN90; WASLBP
NCBI Protein Information
NCK-interacting protein with SH3 domain
UniProt Protein Name
NCK-interacting protein with SH3 domain
Protein Family
UniProt Gene Name
NCKIPSD
UniProt Synonym Gene Names
AF3P21; SPIN90; VIP54; DIP-1; WISH
NCBI Description
The protein encoded by this gene is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation. The gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Uniprot Description
Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.
Research Articles on NCKIPSD
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Product Notes
The NCKIPSD nckipsd (Catalog #AAA9610215) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCKIPSD Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NCKIPSD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the NCKIPSD nckipsd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCKIPSD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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