NCKIPSD recombinant protein

NCKIPSD Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121484

• Gene Names: NCKIPSD; DIP; DIP1; ORF1; WISH; VIP54; AF3P21; SPIN90; WASLBP
• Purity: >90% as determined by SDS-PAGE
• Synonyms: NCKIPSD; NCKIPSD Protein; Human; Recombinant (GST Tag); Human NCKIPSD/SPIN90 Protein (GST Tag); AF3P21 Protein; DIP Protein; DIP1 Protein; ORF1 Protein; SPIN90 Protein; VIP54 Protein; WASLBP Protein; WISH Protein; NCK interacting protein with SH3 domain; NCKIPSD recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr244

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the mature form of human NCKIPSD (Q9NZQ3-3)(Met1-Thr244) was expressed with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for NCKIPSD recombinant protein

Background: NCKIPSD is localized exclusively in the cell nucleus. It plays a role in signal transduction and may function in the maintenance of sarcomeres and the assembly of myofibrils into sarcomeres. NCKIPSD also plays an important role in stress fiber formation. NCKIPSD gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing occurs in this locus and two transcript variants encoding distinct isoforms have been identified. NCKIPSD is an SH3 domain protein. Fas ligand is a cytotoxic effector molecule of T and NK cells which is characterized by an intracellular N-terminal polyproline region that serves as a docking site for SH3 and WW domain proteins. Several previously described Fas ligand-interacting SH3 domain proteins turned out to be crucial for the regulation of storage, expression, and function of the death factor. Recent observations, however, indicate that Fas ligand is also subject to posttranslational modifications including shedding and intramembrane proteolysis.

References

Satoh, S, et al. (2001) mDia-interacting protein acts downstream of Rho-mDia and modifies Src activation and stress fiber formation. J Biol Chem. 276(42):39290-4. de Bernard M, et al. (2000) The VacA toxin of Helicobacter pylori identifies a new intermediate filament-interacting protein. EMBO J. 19(1):48-56. Sano K, et al. (2000) Novel SH3 protein encoded by the AF3p21 gene is fused to the mixed lineage leukemia protein in a therapy-related leukemia with t(3;11)(p21;q23). Blood. 95(3): 1066-8. Voss M, et al. (2009) Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening. BMC Immunol. 10:53.

NCBI and Uniprot Product Information

• NCBI GI #: 7705266
• NCBI GeneID: 51517
• NCBI Accession #: NP_057537
• NCBI GenBank Nucleotide #: NM_016453
• UniProt Accession #: Q9NZQ3; Q6GU34; Q6SPF3; Q8TC10; Q9UGM8; B4DFL5
• Molecular Weight: 64,196 Da
• NCBI Official Full Name: NCK-interacting protein with SH3 domain isoform 1
• NCBI Official Synonym Full Names: NCK interacting protein with SH3 domain
• NCBI Official Symbol: NCKIPSD
• NCBI Official Synonym Symbols: DIP; DIP1; ORF1; WISH; VIP54; AF3P21; SPIN90; WASLBP
• NCBI Protein Information: NCK-interacting protein with SH3 domain; 54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; 90 kDa SH3 protein interacting with Nck; SH3 adapter protein SPIN90; SH3 protein interacting with Nck, 90 kDa; WASP-interacting SH3-domain prote
• UniProt Protein Name: NCK-interacting protein with SH3 domain
• Protein Family: NCK-interacting protein with SH3 domain
• UniProt Gene Name: NCKIPSD
• UniProt Synonym Gene Names: AF3P21; SPIN90; VIP54; DIP-1; WISH
• UniProt Entry Name: SPN90_HUMAN