NCKIPSD / SPIN90 recombinant protein

Recombinant Human NCKIPSD / SPIN90 Protein (GST tag)

Cat. Num. AAA2545273

• Gene Names: NCKIPSD; DIP; DIP1; ORF1; WISH; VIP54; AF3P21; SPIN90; WASLBP
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: NCKIPSD / SPIN90; Recombinant Human NCKIPSD / SPIN90 Protein (GST tag); NCKIPSD; AF3P21; SPIN90; NCKIPSD / SPIN90 recombinant protein

• Host: E Coli
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4
• Sequence Length: 722

• Application Notes: The recombinant human NCKIPSD consists of 478 amino acids and predicts a molecular mass of 53.2 KDa. It migrates as an approximately 53 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for NCKIPSD / SPIN90 recombinant protein

Background: NCKIPSD is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. NCKIPSD also plays an important role in stress fiber formation. NCKIPSD gene is involved in therapy-related leukemia by a chromosomal translocation t (3;11) (p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing occurs in this locus and two transcript variants encoding distinct isoforms have been identified. NCKIPSD is a SH3 domain protein. Fas ligand is a cytotoxic effector molecule of T and NK cells which is characterized by an intracellular N-terminal polyproline region that serves as a docking site for SH3 and WW domain proteins. Several previously described Fas ligand-interacting SH3 domain proteins turned out to be crucial for the regulation of storage, expression and function of the death factor. Recent observations, however, indicate that Fas ligand is also subject to posttranslational modifications including shedding and intramembrane proteolysis.

Description: A DNA sequence encoding the mature form of human NCKIPSD (Q9NZQ3-3) (Met1-Thr244) was expressed with the GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7705266
• NCBI GeneID: 51517
• NCBI Accession #: NP_057537.1
• NCBI GenBank Nucleotide #: NM_016453.3
• UniProt Accession #: Q9NZQ3; Q6GU34; Q6SPF3; Q8TC10; Q9UGM8; B4DFL5
• Molecular Weight: 64,196 Da
• NCBI Official Full Name: NCK-interacting protein with SH3 domain isoform 1
• NCBI Official Synonym Full Names: NCK interacting protein with SH3 domain
• NCBI Official Symbol: NCKIPSD
• NCBI Official Synonym Symbols: DIP; DIP1; ORF1; WISH; VIP54; AF3P21; SPIN90; WASLBP
• NCBI Protein Information: NCK-interacting protein with SH3 domain
• UniProt Protein Name: NCK-interacting protein with SH3 domain
• UniProt Gene Name: NCKIPSD
• UniProt Synonym Gene Names: AF3P21; SPIN90; VIP54; DIP-1; WISH

NCBI Description

The protein encoded by this gene is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation. The gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

NCKIPSD: Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo. In vitro, stimulates N-WASP- induced ARP2/3 complex activation in the absence of CDC42. May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis. A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with MLL. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cytosol; signalosome

Molecular Function: protein binding

Biological Process: NLS-bearing substrate import into nucleus; signal transduction