Rabbit anti-Human NCKAP1L Polyclonal Antibody | anti-NCKAP1L antibody

NCKAP1L Antibody

Cat. Num. AAA9631134

• Gene Names: NCKAP1L; HEM1
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NCKAP1L; Polyclonal Antibody; NCKAP1L Antibody; 4930568P13Rik; AI463083; HEM1; Hematopoietic protein 1; HEMATOPOIETIC PROTEIN HEM-1; Hemp1; Membrane associated protein hem1; Membrane-associated protein HEM-1; NCK associated protein 1 like; Nck-associated protein 1-like; NCKPL_HUMAN; anti-NCKAP1L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: NCKAP1L Antibody detects endogenous levels of NCKAP1L.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NCKAP1L antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human NCKAP1L.
• Conjugation: Unconjugated
• Similarity: Belongs to the HEM-1/HEM-2 family.
• Subunit Structure: In hematopoietic cells, component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1L/HEM1 and WASF2/WAVE2. Interacts with ARHGAP4, PIK3C3/VPS34 and PPP1R12A/MYPT1.
• Tissue Specificity: Expressed only in cells of hematopoietic origin. Expressed in neutrophils (at protein level).
• Function: Essential hematopoietic-specific regulator of the actin cytoskeleton (Probable). Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages. Component of the WAVE2 complex which signals downstream of RAC to stimulate F-actin polymerization. Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells (By similarity). Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin. Also involved in mechanisms WAVE-independent to regulate myosin and actin polymerization during neutrophil chemotaxis.
• Note: Localizes to the leading edge of polarized neutrophils.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-NCKAP1L antibody

Cell membrane; Single-pass membrane protein; Cytoplasmic side; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 34485727
• NCBI GeneID: 3071
• NCBI Accession #: NP_005328.2
• NCBI GenBank Nucleotide #: NM_005337.4
• UniProt Accession #: P55160; Q52LW0; B4DUT5
• Molecular Weight: 122,478 Da
• NCBI Official Full Name: nck-associated protein 1-like isoform 1
• NCBI Official Synonym Full Names: NCK-associated protein 1-like
• NCBI Official Symbol: NCKAP1L
• NCBI Official Synonym Symbols: HEM1
• NCBI Protein Information: nck-associated protein 1-like; hematopoietic protein 1; membrane-associated protein hem-1
• UniProt Protein Name: Nck-associated protein 1-like
• Protein Family: Nck-associated protein
• UniProt Gene Name: NCKAP1L
• UniProt Synonym Gene Names: HEM1
• UniProt Entry Name: NCKPL_HUMAN

NCBI Description

This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]

Uniprot Description

HEM1: a component of the Scar/WAVE complex that plays an important role in regulating cell shape and motility. Found at elevated levels in cells of hematopoietic lineage. Localizes to propagating waves at the leading edge of a motile human neutrophils. The complex stimulates actin assembly; polymerized actin is reciprocally required to remove the complex from the membrane. Overexpression is associated with poor outcome in B-cell chronic lymphocytic leukemia patients.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: membrane; integral to plasma membrane; cytosol

Molecular Function: protein binding; protein complex binding; protein kinase activator activity

Biological Process: response to drug; neutrophil chemotaxis; erythrocyte development; positive regulation of erythrocyte differentiation; myeloid cell homeostasis; positive regulation of cell adhesion mediated by integrin; positive regulation of CD4-positive, alpha beta T cell differentiation; maintenance of cell polarity; chemotaxis; positive regulation of CD8-positive, alpha-beta T cell differentiation; positive regulation of lymphocyte differentiation; B cell receptor signaling pathway; negative regulation of interleukin-17 production; B cell homeostasis; positive regulation of actin filament polymerization; positive regulation of protein kinase activity; negative regulation of interleukin-6 production; positive regulation of B cell proliferation; positive regulation of B cell differentiation; positive regulation of T cell proliferation; T cell homeostasis; protein complex assembly; positive regulation of gamma-delta T cell differentiation; cortical actin cytoskeleton organization and biogenesis; positive regulation of phagocytosis, engulfment; positive regulation of phosphorylation; negative regulation of apoptosis