Rabbit anti-Mouse Cdk4 Polyclonal Antibody | anti-Cdk4 antibody

Cdk4, CT (Cdk4, Crk3, Cyclin-dependent kinase 4, CRK3, Cell division protein kinase 4, PSK-J3) (Azide free) (HRP)

Cat. Num. AAA6284395

• Gene Names: Cdk4; Crk3
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Cdk4; Polyclonal Antibody; CT (Cdk4; Crk3; Cyclin-dependent kinase 4; CRK3; Cell division protein kinase 4; PSK-J3) (Azide free) (HRP); PSK-J3; anti-Cdk4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Cdk4.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Cdk4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 219-246 in the C-terminal region of mouse Cdk4, UniProt Accession #P30285. Species sequence homology: rat
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using 033662. This demonstrates that 033662 detected the Cdk4 protein (arrow).)

Product Categories/Family for anti-Cdk4 antibody

Antibodies; Protein Kinases

References

1. Trivedi, C.M., et al. Dev. Cell 19(3):450-459(2010). 2. Beilke, S., et al. Oncogene 29(28):4058-4067(2010). 3. Puyol, M., et al. Cancer Cell 18(1):63-73(2010). 4. Wiedemeyer, W.R., et al. Proc. Natl. Acad. Sci. U.S.A. 107(25):11501-11506(2010). 5. Michaud, K., et al. Cancer Res. 70(8):3228-3238(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 6753380
• NCBI GeneID: 12567
• NCBI Accession #: NP_034000.1
• NCBI GenBank Nucleotide #: NM_009870.3
• UniProt Accession #: P30285
• Molecular Weight: 33,751 Da
• NCBI Official Full Name: cyclin-dependent kinase 4
• NCBI Official Synonym Full Names: cyclin-dependent kinase 4
• NCBI Official Symbol: Cdk4
• NCBI Official Synonym Symbols: Crk3
• NCBI Protein Information: cyclin-dependent kinase 4; PSK-J3; p34<PSK-J3>/cdk4; serine/threonine kinase; cyclin dependent kinase 4; cell division protein kinase 4
• UniProt Protein Name: Cyclin-dependent kinase 4
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk4
• UniProt Synonym Gene Names: Crk3
• UniProt Entry Name: CDK4_MOUSE

Uniprot Description

CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991.

Protein type: Cell cycle regulation; EC 2.7.11.22; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; CDK family; CDK/CDK4 subfamily; CDK4 subfamily

Cellular Component: transcription factor complex; nuclear membrane; tight junction; membrane; perinuclear region of cytoplasm; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleolus; chromatin; nucleus; cytosol

Molecular Function: transferase activity; protein serine/threonine kinase activity; cyclin binding; protein binding; cyclin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; protein complex binding; kinase activity; ATP binding; protein kinase activity

Biological Process: response to drug; positive regulation of cell size; positive regulation of translation; regulation of cell cycle; positive regulation of apoptosis; response to testosterone stimulus; signal transduction; cell cycle; protein amino acid phosphorylation; regulation of cell proliferation; response to organic substance; positive regulation of fibroblast proliferation; regulation of gene expression; cell division; positive regulation of cell proliferation; response to lead ion; phosphorylation; G1/S transition of mitotic cell cycle