Rabbit anti-Mouse Natural Resistance Associated Macrophage Protein Polyclonal Antibody | anti-Slc11a1 antibody

Natural Resistance Associated Macrophage Protein (Nramp1)

Cat. Num. AAA613061

• Gene Names: Slc11a1; Bcg; Ity; Lsh; ity; Nramp; Nramp1; MGC129156; MGC129157
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Natural Resistance Associated Macrophage Protein; Polyclonal Antibody; Natural Resistance Associated Macrophage Protein (Nramp1); Anti -Natural Resistance Associated Macrophage Protein (Nramp1); anti-Slc11a1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Nramp1.No significant sequence homology exists with Nramp2. Species Sequence Homology: Bison, bovine, ovine, red deer - 59%; Human - 57%.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-Slc11a1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.; Western Blot: 1-10ug/ml (ECL)
• Immunogen: A 21aa peptide sequence located at the predicted cytoplasmic loop near the C-terminus of mouse Nramp1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Slc11a1 antibody

Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Naturally occurring and experimentally induced mutations at Nramp1 abrogate natural resistance to infection. Polymorphic variants at the human Nramp1 locus are associated with differential susceptibility of humans to tuberculosis and leprosy. Nramp1 is expressed in the lysosomal compartment of monocytes and macrophages, lungs, and spleen. It is induced in response to lymphokines and bacterial products. After phagocytosis, Nramp1 is targeted to the membrane of the microbe-containing phagosome, where it may modify the intraphagosomal milieu to affect microbial infection. Although the physiological role of Nramp1 is unclear, structural predictions suggest that Nramp1 encodes the prototypic member of a transporter family. Nramp1 (human 550aa, chromosome 2q35; mouse 548aa) protein is highly hydrophobic integral membrane glycoproteins composed of 12-transmembrane (TM) domains that possess several structural characteristics of ion channels and transporters. Both N- and C-termini are predicted to be cytoplasmic.

Product Categories/Family for anti-Slc11a1 antibody

Antibodies; Abs to Ion Transport

NCBI and Uniprot Product Information

• NCBI GI #: 505156
• NCBI GeneID: 18173
• UniProt Accession #: P41251; Q3TB84
• Molecular Weight: 59,741 Da
• NCBI Official Full Name: natural resistance associated macrophage protein
• NCBI Official Synonym Full Names: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
• NCBI Official Symbol: Slc11a1
• NCBI Official Synonym Symbols: Bcg; Ity; Lsh; ity; Nramp; Nramp1; MGC129156; MGC129157
• NCBI Protein Information: natural resistance-associated macrophage protein 1; NRAMP 1; OTTMUSP00000017968; host resistance locus Bcg/Ity/Lsh
• UniProt Protein Name: Natural resistance-associated macrophage protein 1
• Protein Family: Natural resistance-associated macrophage protein
• UniProt Gene Name: Slc11a1
• UniProt Synonym Gene Names: Bcg; Ity; Lsh; Nramp1
• UniProt Entry Name: NRAM1_MOUSE

Uniprot Description

SLC11A1: Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes. Belongs to the NRAMP family.

Protein type: Transporter, SLC family; Membrane protein, integral; Transporter; Cell surface; Membrane protein, multi-pass; Vesicle

Cellular Component: phagocytic vesicle membrane; cell surface; membrane; lysosome; late endosome; plasma membrane; integral to membrane; vacuole; endosome membrane

Molecular Function: transition metal ion transmembrane transporter activity; protein homodimerization activity; transporter activity; manganese ion transmembrane transporter activity; metal ion:hydrogen antiporter activity

Biological Process: respiratory burst; positive regulation of T-helper 1 type immune response; wound healing; cellular iron ion homeostasis; positive regulation of dendritic cell antigen processing and presentation; manganese ion transport; response to lipopolysaccharide; defense response to Gram-negative bacterium; vacuolar acidification; antigen processing and presentation of peptide antigen; nitrite transport; transport; T cell proliferation during immune response; T cell cytokine production; positive regulation of phagocytosis; interleukin-3 production; inflammatory response; positive regulation of cytokine production; mRNA stabilization; MAPKKK cascade; L-arginine import; defense response to protozoan; iron ion transport; interleukin-2 production; phagocytosis; MHC class II biosynthetic process; iron ion homeostasis; positive regulation of interferon-gamma production; macrophage activation; cellular cadmium ion homeostasis; response to bacterium; protein import into nucleus, translocation; negative regulation of cytokine production; defense response to bacterium; activation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; ion transport