Rabbit anti-Human RBM14 Polyclonal Antibody | anti-RBM14 antibody

Anti-RBM14 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109016

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: RBM14; Polyclonal Antibody; Anti-RBM14 Antibody; Rabbit Polyclonal; RBM14 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-COAA Antibody; Anti-PSP2 Antibody; Anti-SIP Antibody; Anti-SYTIP1 Antibody; Anti-TMEM137 Antibody; anti-RBM14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RBM14
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-RBM14 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human RBM14 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human RBM14 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-RBM14 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:73 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human RBM14 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human RBM14 in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of RBM14 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RBM14 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.)

Immunoprecipitation (IP)

(RBM14 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-RBM14 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RBM14 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa Observed band size :72 kDa)

Related Product Information for anti-RBM14 antibody

RBM14 is a component of the para-speckle complex, which has been implicated in multiple important biological processes. RBM14, is known to function in transcription and RNA splicing. RBM14 is also required for maintaining the stem-like state of GBM spheres, and it controls the DNA-PK-dependent non-homologous end-joining (NHEJ) pathway by interacting with KU80. RBM14 is a RNA-binding protein (RBP) with low complexity domains, called intrinsically disordered proteins (IDPs), and it also physically interacts with PARP1. RBM14 is recruited to DNA double-strand breaks (DSBs) in a poly(ADP-ribose) (PAR)-dependent manner. DNA-dependent PARP1 (poly-(ADP) ribose polymerase 1) makes key contributions in the DNA damage response (DDR) network. RBM14 plays an important role in a PARP-dependent DSB repair process. RBM14 not only plays an important role in maintaining the pluripotency of mouse embryonic stem cells (mESCs) but is also indispensable for mesoderm development.

References

Yuan M, et al. (2014) Rna binding protein rbm14 promotes radio-resistance in glioblastoma by regulating DNA repair and cell differentiation. Oncotarget 5 (9): 2820-2826.Kai M (2016) Roles of rna-binding proteins in DNA damage response. Int J Mol Sci 17 (3): 310.Budhiraja S, et al. (2015) Mining the human complexome database identifies rbm14 as an xpo1-associated protein involved in hiv-1 rev function. J Virol 89 (7): 3557-3567.