Rabbit anti-Human MFN2 Polyclonal Antibody | anti-MFN2 antibody

Anti-MFN2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108388

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: MFN2; Polyclonal Antibody; Anti-MFN2 Antibody; Rabbit Polyclonal; MFN2 Antibody; Rabbit PAb; Antigen Affinity Purified; mitofusin 2; Anti-CMT2A Antibody; Anti-CMT2A2 Antibody; Anti-CPRP1 Antibody; Anti-HSG Antibody; Anti-MARF Antibody; anti-MFN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MFN2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MFN2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human MFN2 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MFN2 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MFN2 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:86 kDa Observed band size:86 kDa (We are unsure as to the identity of these extra bands.))

Immunohistochemistry (IHC)

(Immunochemical staining of human MFN2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of MFN2 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MFN2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Immunoprecipitation (IP)

(MFN2 was immunoprecipitated using: Lane A:0.5 mg HeLa Jurkat Whole Cell Lysate 4 uL anti-MFN2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MFN2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 86 kDa Observed band size :86 kDa)

Related Product Information for anti-MFN2 antibody

Mitofusin 2 (MFN2) is a GTPase dynamin-like protein of the outer mitochondrial membrane, encoded in the nuclear genome by the MFN2 gene located on the short (p) arm of chromosome 1. MFN2 is an important transmembrane GTPase in the mitochondrial outer membrane and it can block cells in the G0/G1 stage of the cell cycle. MFN2 protein is involved in several intracellular pathways but is mainly involved in a network that has an essential role in several mitochondrial functions, including fusion, axonal transport, interorganellar communication, and mitophagy. Mitofusin 2 (Mfn2) is a key protein in mitochondrial fusion and it participates in the bridging of mitochondria to the endoplasmic reticulum (ER). Recent data indicate that Mfn2 ablation leads to ER stress. Mitofusin-2 (MFN2) deficiency in trophoblastic cells is an important cause for early miscarriage. MFN2 can regulate mitochondrial autophagy. Several reports show that MFN2 is a target of different miRNAs.

References

Xu L, et al. (2019) Aberrant mfn2 transcription facilitates homocysteine-induced vsmcs proliferation via the increased binding of c-myc to dnmt1 in atherosclerosis. J Cell Mol Med 23 (7): 4611-4626.Stuppia G, et al. (2015) Mfn2-related neuropathies: Clinical features, molecular pathogenesis and therapeutic perspectives. J Neurol Sci 356 (1-2): 7-18.Munoz JP, et al. (2013) Mfn2 modulates the upr and mitochondrial function via repression of perk. Embo j 32 (17): 2348-2361.Bucha S, et al. (2019) E2f1 activates mfn2 expression by binding to the promoter and decreases mitochondrial fission and mitophagy in hela cells. Febs j 286 (22): 4525-4541.