Rabbit anti-Human, Rat NARP / NPTX2 Polyclonal Antibody | anti-NARP antibody

Rabbit Polyclonal to Human NARP / NPTX2

Cat. Num. AAA248747

• Gene Names: NPTX2; NP2; NARP; NP-II
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: NARP / NPTX2; Polyclonal Antibody; Rabbit Polyclonal to Human NARP / NPTX2; Anti-NARP / NPTX2 Antibody (C-Terminus) IHC-plus; NPTX2; Apexin; Neuronal pentraxin-2; NARP; Neuronal pentraxin II; NP-II; NP2; Human NARP; anti-NARP antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Specificity: This NPTX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the C-terminal region of human NPTX2.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 431

• Applicable Applications for anti-NARP antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P: 1:50; WB: 1:1000; ; Optimal dilution to be determined by researcher.
• Target Species: Human
• Epitope: aa309-338
• Immunogen: NPTX2 antibody was raised against Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Immunohistochemistry (IHC)

(Anti-NARP / NPTX2 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Western Blot (WB)

(Western blot of NARP(Nptx2) (arrow) using rabbit polyclonal NARP(Nptx2) Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NARP(Nptx2) gene (Lane 2).)

NCBI and Uniprot Product Information

• NCBI GI #: 28195384
• NCBI GeneID: 4885
• NCBI Accession #: NP_002514.1
• NCBI GenBank Nucleotide #: NM_002523.2
• UniProt Accession #: P47972; Q86XV7; Q96G70; A4D267
• NCBI Official Full Name: neuronal pentraxin-2
• NCBI Official Synonym Full Names: neuronal pentraxin II
• NCBI Official Symbol: NPTX2
• NCBI Official Synonym Symbols: NP2; NARP; NP-II
• NCBI Protein Information: neuronal pentraxin-2; apexin; neuronal activity-regulated pentaxin
• UniProt Protein Name: Neuronal pentraxin-2
• Protein Family: Nitrate/nitrite response regulator protein
• UniProt Gene Name: NPTX2
• UniProt Synonym Gene Names: NP2; NP-II
• UniProt Entry Name: NPTX2_HUMAN

NCBI Description

This gene encodes a member of the family of neuronal petraxins, synaptic proteins that are related to C-reactive protein. This protein is involved in excitatory synapse formation. It also plays a role in clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. Up-regulation of this gene in Parkinson disease (PD) tissues suggests that the protein may be involved in the pathology of PD. [provided by RefSeq, Feb 2009]

Uniprot Description

NPTX2: Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q21.3-q22.1

Cellular Component: extracellular region

Molecular Function: metal ion binding; carbohydrate binding

Biological Process: synaptic transmission