Rabbit NALP5 Polyclonal Antibody | anti-NLRP5 antibody

NALP5 Antibody

Cat. Num. AAA150106

• Gene Names: NLRP5; MATER; NALP5; PAN11; PYPAF8; CLR19.8
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: NALP5 Antibody is affinity chromatography purified via peptide column.
• Synonyms: NALP5; Polyclonal Antibody; NALP5 Antibody; MATER; PAN11; PYPAF8; CLR19.8; NACHT; LRR and PYD domains-containing protein 5; Mater protein homolog; NLR family; pyrin domain containing 5; anti-NLRP5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NALP5 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1200

• Applicable Applications for anti-NLRP5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: NALP5 antibody can be used for detection of NALP5 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: NALP5 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human NALP5.
• Buffer: NALP5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NALP5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NALP5 in mouse brain tissue lysate with NALP5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of NALP5 in human brain tissue with NALP5 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NALP5 in human brain tissue with NALP5 antibody at 20 μg/mL.)

Related Product Information for anti-NLRP5 antibody

NALP5 Antibody: NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins and are thought to be involved in inflammation and reproduction. NALP5, also known as MATER, is a maternal gene required for early embryonic development in mice. Increased NALP5 expression was observed in two neuronal injury models, and transient expression of recombinant NALP5 in neurons induced caspase-3 activation and apoptosis, suggesting that NALP5 also plays a role in caspase activation and apoptosis in injured neurons, and may thus represent a novel target for therapeutic treatment in neurodegenerative disorders.

NCBI and Uniprot Product Information

• NCBI GI #: 158321897
• NCBI GeneID: 126206
• NCBI Accession #: NP_703148
• NCBI GenBank Nucleotide #: NM_153447.4
• UniProt Accession #: P59047; Q86W29; A8MTY4
• Molecular Weight: 134,342 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 5
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 5
• NCBI Official Symbol: NLRP5
• NCBI Official Synonym Symbols: MATER; NALP5; PAN11; PYPAF8; CLR19.8
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 5; mater protein homolog; maternal antigen that embryos require; NACHT, leucine rich repeat and PYD containing 5; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 5
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 5
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP5
• UniProt Synonym Gene Names: MATER; NALP5
• UniProt Entry Name: NALP5_HUMAN

NCBI Description

The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). Expression of this gene is restricted to the oocyte. A mouse gene that encodes a maternal oocyte protein, similar to this encoded protein, is required for normal early embryogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

NLRP5: As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions. Belongs to the NLRP family.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: protein complex; apical cortex; mitochondrion; nucleolus; cytosol

Molecular Function: ATP binding

Biological Process: regulation of RNA stability; organ morphogenesis; fertilization; cellular protein complex assembly; in utero embryonic development; regulation of protein stability; embryo implantation