Rabbit NAC, alpha Polyclonal Antibody | anti-NACA2 antibody

NAC, alpha (Nascent polypeptide-associated complex) (MaxLight 490)

Cat. Num. AAA6489446

• Gene Names: NACA2; ANAC; NACAL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: NAC; alpha; Polyclonal Antibody; alpha (Nascent polypeptide-associated complex) (MaxLight 490); anti-NACA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the human alpha-NAC protein at ~23kD. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight490.

• Applicable Applications for anti-NACA2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: C-terminal region of human alpha-NAC
• Conjugate: MaxLight490
• Positive Control: Fetal mouse brain, rat brain homogenates and human NGP96 cell lysate.
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot results usingMBS627107. Lane A: fetal mouse brain; Lane B: rat brain homogenate; Lane C: NGP96 cell lysate.)

Related Product Information for anti-NACA2 antibody

Nascent polypeptide-associated complex (NAC) is an abundant cytosolic protein involved in cotranslational targeting of polypeptides to the endoplasmic reticulum (ER) membrane. NAC preparations consist of two copurifying polypeptides termed alpha-and beta-NAC that form a very stable complex in vivo and in vitro, and a functional complex can be reconstituted from the individual subunits. A dissection of the contributions of the individual subunits to NACs function revealed that both subunits are in direct contact with nascent polypeptide chains on the ribosome and that both contribute to the prevention of inappropriate interactions. Alpha-NAC is a coactivator of AP-1-mediated transcription. The alpha-NAC protein functions as a transcriptional coactivator by potentiating the activity of the chimeric Gal4-VP16 activator and of c-Jun homodimers. alpha-NAC binds to and regulates FADD function, which is a critical mediator of signal transduction pathways activated by several members of the TNF receptor gene superfamily. Human alpha-NAC is decreased in patients with Alzheimer's disease and Down syndrome.

Product Categories/Family for anti-NACA2 antibody

Antibodies; Disease Markers

References

1. Beatrix, B., et al., J. Biol. Chem. 275: 37, 838-37, 845 (2000). 2. Moreau, A., et al., Mol. Cell Biol. 18: 1312-1321 (1998). 3. Quelo, I., et al., Biochemistry 43: 2906-2914 (2004). 4. Quelo, I., et al., Gene Expr. 10: 255-262 (2002). 5. Yotov, W.V., et al., Mol. Cell Biol. 18: 1303-1311 (1998). 6. Stilo, R., et al., Biochem. Biophys. Res. Commun. 303: 1034-1041 (2003). 7. Kim, S.H., et al., J. Investig. Med. 50: 293-301 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 40548328
• NCBI GeneID: 342538
• NCBI Accession #: NP_954984.1
• NCBI GenBank Nucleotide #: NM_199290.3
• UniProt Accession #: Q9H009; Q2VIR9
• Molecular Weight: 23,223 Da
• NCBI Official Full Name: nascent polypeptide-associated complex subunit alpha-2
• NCBI Official Synonym Full Names: nascent polypeptide-associated complex alpha subunit 2
• NCBI Official Symbol: NACA2
• NCBI Official Synonym Symbols: ANAC; NACAL
• NCBI Protein Information: nascent polypeptide-associated complex subunit alpha-2; alpha-NAC protein; hom s 2.01 protein; IgE autoantigen alpha-nascent polypeptide-associated complex; nascent polypeptide-associated complex, alpha polypeptide, 2
• UniProt Protein Name: Nascent polypeptide-associated complex subunit alpha-2
• Protein Family: Nascent polypeptide-associated complex
• UniProt Gene Name: NACA2
• UniProt Synonym Gene Names: NACAL; NAC-alpha-like
• UniProt Entry Name: NACA2_HUMAN

Uniprot Description

NACA2: Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). Belongs to the NAC-alpha family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: cytoplasm; nucleus

Biological Process: protein transport