Rabbit NAC, alpha Polyclonal Antibody | anti-Nacalpha antibody

NAC, alpha (Nascent polypeptide-associated complex)

Cat. Num. AAA627107

• Gene Names: Nacalpha; aic; alpha-NAC; alphaNAC; CG8759; DmelCG8759; l(2)04329; NAC; NACalpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NAC; alpha; Polyclonal Antibody; alpha (Nascent polypeptide-associated complex); Anti -NAC; anti-Nacalpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the human alpha-NAC protein at ~23kD. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-Nacalpha antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-2ug/ml
• Immunogen: Synthetic peptide derived from the C-terminal region (between aa171-215) of the human alpha-NAC protein.
• Positive Control: Fetal mouse brain, rat brain homogenates and human NGP96 cell lysate.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot results using MBS627107. Lane A: fetal mouse brain; Lane B: rat brain homogenate; Lane C: NGP96 cell lysate.)

Related Product Information for anti-Nacalpha antibody

Nascent polypeptide-associated complex (NAC) is an abundant cytosolic protein involved in cotranslational targeting of polypeptides to the endoplasmic reticulum (ER) membrane. NAC preparations consist of two copurifying polypeptides termed alpha- and beta-NAC that form a very stable complex in vivo and in vitro, and a functional complex can be reconstituted from the individual subunits. A dissection of the contributions of the individual subunits to NACs function revealed that both subunits are in direct contact with nascent polypeptide chains on the ribosome and that both contribute to the prevention of inappropriate interactions. Alpha-NAC is a coactivator of AP-1-mediated transcription. The alpha-NAC protein functions as a transcriptional coactivator by potentiating the activity of the chimeric Gal4-VP16 activator and of c-Jun homodimers. alpha-NAC binds to and regulates FADD function, which is a critical mediator of signal transduction pathways activated by several members of the TNF receptor gene superfamily. Human alpha-NAC is decreased in patients with Alzheimer's disease and Down syndrome.

Product Categories/Family for anti-Nacalpha antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 24653200
• NCBI GeneID: 36409
• NCBI Accession #: NP_725229.1
• NCBI GenBank Nucleotide #: NM_165950.1
• UniProt Accession #: Q94518; O16813; Q0E999; A4UZF1
• Molecular Weight: 23,021 Da
• NCBI Official Full Name: nascent polypeptide associated complex protein alpha subunit, isoform C
• NCBI Official Synonym Full Names: Nascent polypeptide associated complex protein alpha subunit
• NCBI Official Symbol: Nacalpha
• NCBI Official Synonym Symbols: aic; alpha-NAC; alphaNAC; CG8759; DmelCG8759; l(2)04329; NAC; NACalpha
• NCBI Protein Information: CG8759 gene product from transcript CG8759-RB; CG8759-PA; CG8759-PB; CG8759-PC; NAC protein alpha subunit; Nacalpha-PA; Nacalpha-PB; Nacalpha-PC; alpha NAC; lethal (2) 04329; nascent polypeptide associated complex protein alpha subunit
• UniProt Protein Name: Nascent polypeptide-associated complex subunit alpha
• UniProt Gene Name: Nacalpha
• UniProt Synonym Gene Names: aic; NAC-alpha
• UniProt Entry Name: NACA_DROME

Uniprot Description

Function: May promote appropriate targeting of ribosome-nascent polypeptide complexes

By similarity. Required for correct localization of the osk/oskar protein to the posterior pole during embryonic development. The osk protein directs the recruitment of molecules responsible for posterior body patterning and germline formation in the embryo. Ref.6

Subunit structure: Part of the nascent polypeptide-associated complex (NAC), consisting of Nac-alpha and bicaudal (bic).

Sequence similarities: Belongs to the NAC-alpha family.Contains 1 NAC-A/B (NAC-alpha/beta) domain.Contains 1 UBA domain.