Mouse anti-Human Lissencephaly-1 Monoclonal Antibody | anti-Lis-1 antibody

Lissencephaly-1 (LIS-1, MDCR, Miller-Dieker Syndrome, MDS, PAF Acetylhydrolase 45kD Subunit, PAFAH 45kD, PAFAH alpha, PAFAH1B1, Platelet Activating Factor Acetylhydrolase Isoform Ib alpha Subunit, PAFAHA, SBH, Subcortical Band Heterotopia, ILS, Isolated L

Cat. Num. AAA608234

• Gene Names: Lis-1; CG8440; D-Lis1; Dlis-1; DLis-1; DLIS-1; dLis1; Dlis1; DLis1; DLIS1; DmelCG8440; DmLIS1; l(2)k13209; l(2R)W8; Lis 1; lis-1; LIS-1; lis1; Lis1; n(2)k11702
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Lissencephaly-1; Monoclonal Antibody; Lissencephaly-1 (LIS-1; MDCR; Miller-Dieker Syndrome; MDS; PAF Acetylhydrolase 45kD Subunit; PAFAH 45kD; PAFAH alpha; PAFAH1B1; Platelet Activating Factor Acetylhydrolase Isoform Ib alpha Subunit; PAFAHA; SBH; Subcortical Band Heterotopia; ILS; Isolated L; Anti -Lissencephaly-1 (LIS-1; anti-Lis-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9G377
• Specificity: Recognizes human Lissencephaly-1.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

• Applicable Applications for anti-Lis-1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.
• Immunogen: Partial recombinant human PAFAH alpha (1-111) with a GST tag.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot detection of GST tagged recombinant human PAFAH alpha. Note the GST tag alone has a molecular weight of 26kD.)

Related Product Information for anti-Lis-1 antibody

PAFAH alpha encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. It is expressed ubiquitously in both the frontal and occipital areas of the brain. Defects in PAFAH alpha cause lissencephaly type 1 (LIS1) and of subcortical band heterotopia (SBH). Defects in PAFAH alpha are also a cause of Miller-Dieker lissencephaly syndrome (MLDS).

Product Categories/Family for anti-Lis-1 antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 45552655
• NCBI GeneID: 36791
• NCBI Accession #: NP_995852.1
• NCBI GenBank Nucleotide #: NM_206130.1
• UniProt Accession #: Q7KNS3; O96698; Q5BI20; Q8SXJ6; A4UZJ3
• Molecular Weight: 46,460 Da
• NCBI Official Full Name: Lissencephaly-1, isoform F
• NCBI Official Synonym Full Names: Lissencephaly-1
• NCBI Official Symbol: Lis-1
• NCBI Official Synonym Symbols: CG8440; D-Lis1; Dlis-1; DLis-1; DLIS-1; dLis1; Dlis1; DLis1; DLIS1; DmelCG8440; DmLIS1; l(2)k13209; l(2R)W8; Lis 1; lis-1; LIS-1; lis1; Lis1; n(2)k11702
• NCBI Protein Information: CG8440 gene product from transcript CG8440-RE; Lissencephaly-1, isoform E; CG8440-PE; Lis-1-PE; lissencephaly-1; Paf-AHbeta; platelet-activating factor acetylhydrolase Ib beta-subunit homolog; Lissencephaly-1, isoform D; CG8440-PD; Lis-1-PD; Lissencephaly-1, isoform A; CG8440-PA; Lis-1-PA; Lissencephaly-1, isoform C; CG8440-PC; Lis-1-PC; Lissencephaly-1, isoform B; CG8440-PB; Lis-1-PB; Lissencephaly-1, isoform F; CG8440-PF; Lis-1-PF
• UniProt Protein Name: Lissencephaly-1 homolog
• Protein Family: Lissencephaly
• UniProt Gene Name: Lis-1
• UniProt Synonym Gene Names: Lis1
• UniProt Entry Name: LIS1_DROME

Uniprot Description

Function: Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as nuclear migration during cell division, mitotic spindle formation and the removal of mitotic checkpoint proteins from kinetochores at the metaphase to anaphase transition. Required for several aspects of neurogenesis including neuroblast proliferation, neuronal cell differentiation, dendritic growth, branching and maturation and axonal transport. Required for synchronized cell divisions in the germline, fusome integrity and oocyte differentiation. Acts together with BicD, Egl, dynein and microtubules to determine oocyte identity during oogenesis. Also required for nurse cell to oocyte transport during oocyte growth and for the positioning of the nucleus in the oocyte. Ref.1 Ref.2 Ref.8 Ref.9 Ref.10 Ref.11

Subunit structure: Interacts with dynein and dynactin. Does not interact with Paf-A-Halpha. Ref.3 Ref.11

Subcellular location: Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton › centrosome

By similarity. Cytoplasm › cytoskeleton › spindle pole. Chromosome › centromere › kinetochore. Note: Localizes to the plus end of microtubules. Also localizes to the mitotic spindle poles from late prophase through to telophase and to kinetochore microtubules in metaphase. Localization to kinetochore microtubules is reduced in anaphase and telophase. In oocytes, it concentrates in the cortex from stage 5 of oogenesis. Ref.2 Ref.11

Tissue specificity: Expressed primarily in germline cells during oogenesis. Low levels are detected in the germarium in regions 1 and 2 and in both nurse cells and the oocyte in stage 2-4 egg chambers. Enriched in the oocyte during stages 5-7 and in nurse cells in stage 8-10 egg chambers. Ubiquitously distributed in early embryos. Expressed throughout cell bodies, dendrites and axons of the mushroom-body neurons. Ref.1 Ref.8 Ref.9

Developmental stage: Expressed both maternally and zygotically. Expressed throughout all developmental stages but is most abundant at precellular blastoderm stages; expression declines after gastrulation. Expressed at higher level in adult females. Following germ-band retraction it is enriched in the developing central nervous system. In third-instar larvae, low levels are detected in the brain hemispheres and imaginal disks. Ref.8

Domain: Dimerization mediated by the LisH domain may be required to activate dynein

Sequence similarities: Belongs to the WD repeat LIS1/nudF family.Contains 1 LisH domain.Contains 7 WD repeats.

Sequence caution: The sequence AAC83821.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.