Rabbit Na+ CP type I alpha Polyclonal Antibody | anti-SCN1A antibody

Na+ CP type I alpha Antibody

Cat. Num. AAA9608893

• Gene Names: SCN1A; FEB3; FHM3; NAC1; SCN1; SMEI; EIEE6; FEB3A; HBSCI; GEFSP2; Nav1.1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: Na+ CP type I alpha; Polyclonal Antibody; Na+ CP type I alpha Antibody; HBSC II; HH1; hNaN; hNE-Na; hPN3; KIAA1356; MED; NAC1; NAC2; NAC3; NENA; Neuroendocrine sodium channel; Peripheral nerve sodium channel 3; Peripheral nerve sodium channel 5; Peripheral sodium channel 1; PN1; PN3; PN5; SCN1; SCN10A; SCN11A; SCN12A; SCN1A; SCN2A; SCN2A1; SCN2A2; SCN3A; SCN4A; SCN5A; SCN8A; SCN9A; Sensory neuron sodium channel 2; SkM1; SNS2; Sodium channel protein brain I subunit alpha; Sodium channel protein brain II subunit alpha; Sodium channel protein brain III subunit alpha; Sodium channel protein cardiac muscle subunit alpha; Sodium channel protein skeletal muscle subunit alpha; Sodium channel protein type 1 subunit alpha; Sodium channel protein type 10 subunit alpha; Sodium channel protein type 11 subunit alpha; Sodium channel protein type 2 subunit alpha; Sodium channel protein type 3 subunit alpha; Sodium channel protein type 4 subunit alpha; Sodium channel protein type 5 subunit alpha; Sodium channel protein type 8 subunit alpha; Sodium channel protein type 9 subunit alpha; Sodium channel protein type II subunit alpha; Sodium channel protein type III subunit alpha; Sodium channel protein type IV subunit alpha; Sodium channel protein type IX subunit alpha; Sodium channel protein type V subunit alpha; Sodium channel protein type VIII subunit alpha; Sodium channel protein type X subunit alpha; Sodium channel protein type XI subunit alpha; Voltage-gated sodium channel subtype III; Voltage-gated sodium channel subunit alpha Nav1.1; Voltage-gated sodium channel subunit alpha Nav1.2; Voltage-gated sodium channel subunit alpha Nav1.3; Voltage-gated sodium channel subunit alpha Nav1.4; Voltage-gated sodium channel subunit alpha Nav1.5; Voltage-gated sodium channel subunit alpha Nav1.6; Voltage-gated sodium channel subunit alpha Nav1.7; Voltage-gated sodium channel subunit alpha Nav1.8; Voltage-gated sodium channel subunit alpha Nav1.9; anti-SCN1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Na+ CP type I alpha antibody detects endogenous levels of total Na+ CP type I alpha
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 2009

• Applicable Applications for anti-SCN1A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human Na+ CP type I alpha
• Subcellular Location: Membrane.
• Predicted Cross Reactivity: Bovine, Horse, Sheep, Rabbit, Chicken
• Similarity: Bovine (100%), Horse (80%), Sheep (87%), Rabbit (100%), Chicken (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis Na+ CP type I alpha using HepG2 whole cell lysates)

Related Product Information for anti-SCN1A antibody

Function: Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. Plays a key role in brain, probably by regulating the moment when neurotransmitters are released in neurons. Involved in sensory perception of mechanical pain: activation in somatosensory neurons induces pain without neurogenic inflammation and produces hypersensitivity to mechanical, but not thermal stimuli.
Subunit Structure: The voltage-sensitive sodium channel consists of an ion conducting pore forming alpha-subunit regulated by one or more beta-1 (SCN1B), beta-2 (SCN2B), beta-3 (SCN3B) and/or beta-4 (SCN4B). Beta-1 (SCN1B) and beta-3 (SCN3B) are non-covalently associated with alpha, while beta-2 (SCN2B) and beta-4 (SCN4B) are covalently linked by disulfide bonds. Interacts with FGF13 (PubMed:21566136). Interacts with SCN1B (PubMed:17928445, PubMed:15525788). Interacts with the conotoxin GVIIJ (PubMed:24497506). Interacts with the spider beta/delta-theraphotoxin-Pre1a (PubMed:28428547).
Post-translational Modifications: Phosphorylation at Ser-1516 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
Similarity: The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.The S3b-S4 and S1-S2 loops of repeat IV are targeted by H.maculata toxins Hm1a and Hm1b, leading to inhibit fast inactivation of Nav1.1/SCN1A. Selectivity for H.maculata toxins Hm1a and Hm1b depends on S1-S2 loops of repeat IV. Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.1/SCN1A subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 260166633
• NCBI GeneID: 6323
• NCBI Accession #: NP_001159435.1
• NCBI GenBank Nucleotide #: NM_001165963.2
• UniProt Accession #: P35498; Q16172; Q585T7; Q8IUJ6; Q96LA3; Q9C008; E9PG49
• Molecular Weight: Observed: 228 kDa; Predicted: 229 kDa
• NCBI Official Full Name: sodium channel protein type 1 subunit alpha isoform 1
• NCBI Official Synonym Full Names: sodium voltage-gated channel alpha subunit 1
• NCBI Official Symbol: SCN1A
• NCBI Official Synonym Symbols: FEB3; FHM3; NAC1; SCN1; SMEI; EIEE6; FEB3A; HBSCI; GEFSP2; Nav1.1
• NCBI Protein Information: sodium channel protein type 1 subunit alpha
• UniProt Protein Name: Sodium channel protein type 1 subunit alpha
• Protein Family: Sodium channel protein
• UniProt Gene Name: SCN1A
• UniProt Synonym Gene Names: NAC1; SCN1

NCBI Description

Voltage-dependent sodium channels are heteromeric complexes that regulate sodium exchange between intracellular and extracellular spaces and are essential for the generation and propagation of action potentials in muscle cells and neurons. Each sodium channel is composed of a large pore-forming, glycosylated alpha subunit and two smaller beta subunits. This gene encodes a sodium channel alpha subunit, which has four homologous domains, each of which contains six transmembrane regions. Allelic variants of this gene are associated with generalized epilepsy with febrile seizures and epileptic encephalopathy. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons, the exact combination of which have not been experimentally confirmed for the full-length transcript. [provided by RefSeq, Oct 2015]

Uniprot Description

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. Plays a key role in brain, probably by regulating the moment when neurotransmitters are released in neurons. Involved in sensory perception of mechanical pain: activation in somatosensory neurons induces pain without neurogenic inflammation and produces hypersensitivity to mechanical, but not thermal stimuli.