Rabbit Na+ CP type VIII alpha Polyclonal Antibody | anti-SCN8A antibody

Na+ CP type VIII alpha Antibody

Cat. Num. AAA9608894

• Gene Names: SCN8A; MED; PN4; CIAT; BFIS5; NaCh6; CERIII; EIEE13; Nav1.6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: Na+ CP type VIII alpha; Polyclonal Antibody; Na+ CP type VIII alpha Antibody; CerIII; CIAT; EIEE13; hNa6/Scn8a voltage gated sodium channel; MED; Motor endplate disease; NaCh 6; NaCh6; Nav 1.6; Nbna1; peripheral nerve protein type 4; PN 4; PN4; SCN8A; SCN8A_HUMAN; Sodium channel protein type 8 alpha subunit; Sodium channel protein type 8 subunit alpha; Sodium channel protein type VIII alpha subunit; Sodium channel protein type VIII subunit alpha; Sodium channel voltage gated type VIII alpha; Sodium channel voltage gated type VIII alpha polypeptide; Sodium channel voltage gated type VIII alpha subunit; Voltage gated sodium channel subunit alpha Nav1.6; Voltage-gated sodium channel subunit alpha Nav1.6; anti-SCN8A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Na+ CP type VIII alpha antibody detects endogenous levels of total Na+ CP type VIII alpha
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1939

• Applicable Applications for anti-SCN8A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human Na+ CP type VIII alpha
• Subcellular Location: Membrane and Cytoplasmic Vesicle. Some vesicles are localized adjacent to melanoma invadopodia and macrophage podosomes. Does not localize to the plasma membrane.
• Tissue Specificity: Isoform 5 is expressed in non-neuronal tissues, such as monocytes/macrophages.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Similarity: Pig (94%), Bovine (94%), Horse (94%), Sheep (94%), Rabbit (88%), Dog (94%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis Na+ CP type VIII alpha using LOVO whole cell lysates)

Related Product Information for anti-SCN8A antibody

Function: Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. In macrophages and melanoma cells, isoform 5 may participate in the control of podosome and invadopodia formation.
Subunit Structure: The voltage-sensitive sodium channel consists of an ion conducting pore forming alpha-subunit regulated by one or more beta-1 (SCN1B), beta-2 (SCN2B), beta-3 (SCN3B) and/or beta-4 (SCN4B). Beta-1 (SCN1B) and beta-3 (SCN3B) are non-covalently associated with alpha, while beta-2 (SCN2B) and beta-4 (SCN4B) are covalently linked by disulfide bonds. Interacts with NEDD4 and NEDD4L (By similarity). Interacts with FGF13. Interacts with FGF14, GBG3, GBB2 and SCN1B (PubMed:26900580). Interacts with the conotoxin GVIIJ (PubMed:24497506). Interacts with the conotoxin GVIIJ (PubMed:24497506). Interacts with the spider beta/delta-theraphotoxin-Pre1a (PubMed:28428547).
Post-translational Modifications: May be ubiquitinated by NEDD4L; which would promote its endocytosis. Phosphorylation at Ser-1497 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
Similarity: The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 295789135
• NCBI GeneID: 6334
• NCBI Accession #: NP_001171455.1
• NCBI GenBank Nucleotide #: NM_001177984.2
• UniProt Accession #: Q9UQD0; O95788; Q9NYX2; Q9UPB2; B9VWG8
• Molecular Weight: Observed: 225 kDa; Predicted: 226 kDa
• NCBI Official Full Name: sodium channel protein type 8 subunit alpha isoform 2
• NCBI Official Synonym Full Names: sodium voltage-gated channel alpha subunit 8
• NCBI Official Symbol: SCN8A
• NCBI Official Synonym Symbols: MED; PN4; CIAT; BFIS5; NaCh6; CERIII; EIEE13; Nav1.6
• NCBI Protein Information: sodium channel protein type 8 subunit alpha
• UniProt Protein Name: Sodium channel protein type 8 subunit alpha
• Protein Family: Sodium channel protein
• UniProt Gene Name: SCN8A
• UniProt Synonym Gene Names: MED

NCBI Description

This gene encodes a member of the sodium channel alpha subunit gene family. The encoded protein forms the ion pore region of the voltage-gated sodium channel. This protein is essential for the rapid membrane depolarization that occurs during the formation of the action potential in excitable neurons. Mutations in this gene are associated with cognitive disability, pancerebellar atrophy and ataxia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. In macrophages and melanoma cells, isoform 5 may participate in the control of podosome and invadopodia formation.