Rabbit SCN4A Polyclonal Antibody | anti-SCN4A antibody

SCN4A Antibody

Cat. Num. AAA9605967

• Gene Names: SCN4A; HYPP; SkM1; CMS16; HYKPP; NAC1A; HOKPP2; Nav1.4; Na(V)1.4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: SCN4A; Polyclonal Antibody; SCN4A Antibody; HYKPP; HYPP; Na(V)1.4; NAC1A; Nav1.4; Scn4a; SCN4A_HUMAN; Skeletal muscle voltage dependent sodium channel type IV alpha subunit; SkM1; Sodium channel protein skeletal muscle subunit alpha; Sodium channel protein type 4 subunit alpha; Sodium channel protein type IV subunit alpha; Sodium channel voltage gated type IV alpha subunit; Voltage gated sodium channel subunit alpha Nav1.4; Voltage gated sodium channel type 4 alpha; Voltage-gated sodium channel subunit alpha Nav1.4; anti-SCN4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SCN4A antibody detects endogenous levels of total SCN4A
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1836

• Applicable Applications for anti-SCN4A antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Membrane.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Similarity: Pig (88%), Bovine (100%), Horse (86%), Sheep (89%), Rabbit (89%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from K562 cells, using SCN4A antibody.)

Immunofluorescene (IF)

(MBS9605967 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-SCN4A antibody

Function: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle.
Subunit Structure: Muscle sodium channels contain an alpha subunit and a smaller beta subunit. Heterooligomer with SCN2B or SCN4B; disulfide-linked. Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2. Interacts with the conotoxin GVIIJ (PubMed:24497506).
Post-translational Modifications: Phosphorylation at Ser-1328 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
Similarity: The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.4/SCN4A subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 93587342
• NCBI GeneID: 6329
• NCBI Accession #: NP_000325.4
• NCBI GenBank Nucleotide #: NM_000334.4
• UniProt Accession #: P35499; Q15478; Q16447; Q7Z6B1
• Molecular Weight: Observed: 208 kD; Predicted: 209 kDa
• NCBI Official Full Name: sodium channel protein type 4 subunit alpha
• NCBI Official Synonym Full Names: sodium voltage-gated channel alpha subunit 4
• NCBI Official Symbol: SCN4A
• NCBI Official Synonym Symbols: HYPP; SkM1; CMS16; HYKPP; NAC1A; HOKPP2; Nav1.4; Na(V)1.4
• NCBI Protein Information: sodium channel protein type 4 subunit alpha
• UniProt Protein Name: Sodium channel protein type 4 subunit alpha
• Protein Family: Sodium channel protein
• UniProt Gene Name: SCN4A

NCBI Description

Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family. It is expressed in skeletal muscle, and mutations in this gene have been linked to several myotonia and periodic paralysis disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle.