Rabbit N Polyclonal Antibody | anti-N antibody

N Antibody

Cat. Num. AAA7049807

• Reactivity: Junin mammarenavirus
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G purified
• Synonyms: N; Polyclonal Antibody; N Antibody; Nucleoprotein; Nucleocapsid protein; Protein N; anti-N antibody

• Host: Rabbit
• Reactivity: Junin mammarenavirus
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 564

• Applicable Applications for anti-N antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1: 500-1: 5000
• Immunogen: Recombinant Junin mammarenavirus Nucleoprotein protein (1-564AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in Recombinant proteinAll lanes: N antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 64 KDaobserved band size: 64 KDa)

Related Product Information for anti-N antibody

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3 phosphorylation and activation by host IKBKE. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of IRF3, a protein involved in IFN activation pathway, leading to the inhibition of IFNB and IRF3-dependent promoters activation (By similarity). Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 127893
• UniProt Accession #: P14239
• Molecular Weight: 63,027 Da
• NCBI Official Full Name: Nucleoprotein
• UniProt Protein Name: Nucleoprotein
• Protein Family: NIP3
• UniProt Gene Name: N
• UniProt Entry Name: NCAP_JUNIN

Uniprot Description

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3 phosphorylation and activation by host IKBKE. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of IRF3, a protein involved in IFN activation pathway, leading to the inhibition of IFNB and IRF3-dependent promoters activation (). Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.