Rabbit anti-Mouse Mylk3 Polyclonal Antibody | anti-Mylk3 antibody

Mylk3, NT (Mylk3, Putative myosin light chain kinase 3) (PE)

Cat. Num. AAA6323406

• Gene Names: Mylk3; MLCK; D830007F02Rik
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Mylk3; Polyclonal Antibody; NT (Mylk3; Putative myosin light chain kinase 3) (PE); Putative myosin light chain kinase 3; anti-Mylk3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Mylk3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Mylk3 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 174-203 in the N-terminal region of mouse Mylk3, UniProt Accession #NP_780650.2; Q3UIZ8.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse heart lysates (20ug) using MBS646079 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution Predicted band size: 86.4kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Mylk3 antibody

Mylk3 may play a role in smooth muscle contraction (By similarity).

Product Categories/Family for anti-Mylk3 antibody

Antibodies; Protein Kinases

References

1. White, R.A., et al. Blood Cells Mol. Dis. 43(2):141-148(2009). 2. Chan, J.Y., et al. Circ. Res. 102(5):571-580(2008). 3. Kim, B.J., et al. Can. J. Physiol. Pharmacol. 85(12):1254-1262(2007). 4. Seguchi, O., et al. J. Clin. Invest. 117(10):2812-2824(2007). 5. Choi, Y.O., et al. Exp. Mol. Med. 38(2):180-189(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 83776559
• NCBI GeneID: 213435
• NCBI Accession #: NP_780650.2
• NCBI GenBank Nucleotide #: NM_175441.5
• UniProt Accession #: Q3UIZ8; Q3V3V0; Q8BWD1; B0LY41
• Molecular Weight: 86,372 Da
• NCBI Official Full Name: myosin light chain kinase 3 isoform 1
• NCBI Official Synonym Full Names: myosin light chain kinase 3
• NCBI Official Symbol: Mylk3
• NCBI Official Synonym Symbols: MLCK; D830007F02Rik
• NCBI Protein Information: myosin light chain kinase 3; cardiac MLCK; cardiac-MLCK; putative myosin light chain kinase 3; cardiac-MyBP-C-associated Ca/CaM kinase; cardiac-specific myosin light chain kinase
• UniProt Protein Name: Myosin light chain kinase 3
• Protein Family: Myosin light chain kinase
• UniProt Gene Name: Mylk3
• UniProt Synonym Gene Names: Cardiac-MLCK
• UniProt Entry Name: MYLK3_MOUSE

Uniprot Description

caMLCK: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.18; Protein kinase, CAMK; CAMK group; MLCK family

Cellular Component: cytoplasm; cytosol; actin cytoskeleton

Molecular Function: transferase activity; protein serine/threonine kinase activity; calmodulin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity; myosin light chain kinase activity

Biological Process: sarcomerogenesis; cardiac myofibril assembly; sarcomere organization; regulation of vascular permeability during acute inflammatory response; phosphorylation; protein amino acid phosphorylation