Western Blot (WB)
(Western Blot analysis in mouse heart lysates (20ug) using MBS646079 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution Predicted band size: 86.4kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Mouse Mylk3 Polyclonal Antibody | anti-Mylk3 antibody
Mylk3, NT (Mylk3, Putative myosin light chain kinase 3)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(Western Blot analysis in mouse heart lysates (20ug) using MBS646079 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution Predicted band size: 86.4kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in mouse heart lysates (20ug) using MBS646079 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution Predicted band size: 86.4kD Blocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Calmodulin-dependent kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes. Increases cardiomyocyte contractility. Ref.1 Ref.3
Catalytic activity: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.
Cofactor: Magnesium
By similarity.
Subcellular location: Cytoplasm Ref.3.
Tissue specificity: Expressed in cardiomyocytes (at protein level). Up-regulated in heart after experimental myocardial infarction at the mRNA level. Ref.1 Ref.3
Developmental stage: Up-regulated in the heart from 1 week after birth through adulthood. Ref.3
Induction: by NRG1 (at protein level). Ref.1
Post-translational modification: Phosphorylated on serine residues
By similarity.
Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.Contains 1 protein kinase domain.
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Product Notes
The Mylk3 mylk3 (Catalog #AAA646079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mylk3, NT (Mylk3, Putative myosin light chain kinase 3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mylk3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the Mylk3 mylk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mylk3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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