Rabbit anti-Human MYLK3 Polyclonal Antibody | anti-MYLK3 antibody

MYLK3, ID (MYLK3, MLCK, Putative myosin light chain kinase 3, Cardiac-MyBP-C-associated Ca/CaM kinase) (MaxLight 650)

Cat. Num. AAA6323393

• Gene Names: MYLK3; MLCK; MLCK2; caMLCK
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYLK3; Polyclonal Antibody; ID (MYLK3; MLCK; Putative myosin light chain kinase 3; Cardiac-MyBP-C-associated Ca/CaM kinase) (MaxLight 650); Cardiac-MyBP-C-associated Ca/CaM kinase; anti-MYLK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-MYLK3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MYLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 542-570 amino acids from the Central region of human MYLK3.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-MYLK3 antibody

Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).

Product Categories/Family for anti-MYLK3 antibody

Antibodies; Protein Kinases

References

Al-Sadi, R., et al. J. Immunol. 180(8):5653-5661(2008). Chan, J.Y., et al. Circ. Res. 102(5):571-580(2008). Seguchi, O., et al. J. Clin. Invest. 117(10):2812-2824(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 146219832
• NCBI GeneID: 91807
• NCBI Accession #: NP_872299.2
• NCBI GenBank Nucleotide #: NM_182493.2
• UniProt Accession #: Q32MK0; Q32MK1; Q96DV1; B5BUL9; B7Z5U8
• Molecular Weight: 52,723 Da
• NCBI Official Full Name: myosin light chain kinase 3
• NCBI Official Synonym Full Names: myosin light chain kinase 3
• NCBI Official Symbol: MYLK3
• NCBI Official Synonym Symbols: MLCK; MLCK2; caMLCK
• NCBI Protein Information: myosin light chain kinase 3; MLC kinase; cardiac-MLCK; putative myosin light chain kinase 3; cardiac-MyBP-C associated Ca/CaM kinase; cardiac-MyBP-C-associated Ca/CaM kinase
• UniProt Protein Name: Myosin light chain kinase 3
• Protein Family: Myosin light chain kinase
• UniProt Gene Name: MYLK3
• UniProt Synonym Gene Names: MLCK; Cardiac-MLCK

Uniprot Description

caMLCK: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: CAMK group; EC 2.7.11.18; Kinase, protein; MLCK family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16q11.2

Cellular Component: actin cytoskeleton; cytoplasm; cytosol; intracellular

Molecular Function: ATP binding; calmodulin-dependent protein kinase activity; myosin light chain kinase activity

Biological Process: cardiac myofibril assembly; cellular response to interleukin-1; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; protein amino acid phosphorylation; regulation of vascular permeability during acute inflammatory response; sarcomere organization; sarcomerogenesis