Porcine Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5 ELISA Kit | NOX5 elisa kit

Porcine Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5 ELISA Kit

Cat. Num. AAA755240

• Reactivity: Porcine
• Synonyms: Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5; Porcine Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5 ELISA Kit; NOX5 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOX5 elisa kit

Intended Uses: This NOX5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine NOX5. This ELISA kit for research use only!

Principle of the Assay: NOX5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NOX5 antibody and an NOX5-HRP conjugate. The assay sample and buffer are incubated together with NOX5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NOX5 concentration since NOX5 from samples and NOX5-HRP conjugate compete for the anti-NOX5 antibody binding site. Since the number of sites is limited, as more sites are occupied by NOX5 from the sample, fewer sites are left to bind NOX5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NOX5 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 296278231
• NCBI GeneID: 79400
• NCBI Accession #: NP_001171709.1
• NCBI GenBank Nucleotide #: NM_001184780.1
• Molecular Weight: 83,109 Da
• NCBI Official Full Name: NADPH oxidase 5 isoform 3
• NCBI Official Synonym Full Names: NADPH oxidase, EF-hand calcium binding domain 5
• NCBI Official Symbol: NOX5
• NCBI Protein Information: NADPH oxidase 5
• UniProt Protein Name: NADPH oxidase 5
• Protein Family: NADPH oxidase
• UniProt Gene Name: NOX5
• UniProt Entry Name: NOX5_HUMAN

NCBI Description

This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

NOX5: Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.6.3.-; Oxidoreductase; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; FAD binding; superoxide-generating NADPH oxidase activity; heme binding; calcium ion binding; hydrogen ion channel activity; NADP binding

Biological Process: cell proliferation; proton transport; regulation of fusion of sperm to egg plasma membrane; apoptosis; endothelial cell proliferation; cytokinesis; regulation of proton transport; angiogenesis; cytokine secretion; superoxide release