Western Blot (WB)
(MYLK3 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MYLK3 antibody detected the MYLK3 protein (arrow).)
Rabbit anti-Human MYLK3 Polyclonal Antibody | anti-MYLK3 antibody
MYLK3, ID (MYLK3, MLCK, Putative myosin light chain kinase 3, Cardiac-MyBP-C-associated Ca/CaM kinase) (Azide free) (HRP)
Gene Names
MYLK3; MLCK; MLCK2; caMLCK
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MYLK3; Polyclonal Antibody; ID (MYLK3; MLCK; Putative myosin light chain kinase 3; Cardiac-MyBP-C-associated Ca/CaM kinase) (Azide free) (HRP); Cardiac-MyBP-C-associated Ca/CaM kinase; anti-MYLK3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-MYLK3 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
MYLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 542-570 amino acids from the Central region of human MYLK3.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MYLK3 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MYLK3 antibody detected the MYLK3 protein (arrow).)
Western Blot (WB)
(MYLK3 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MYLK3 antibody detected the MYLK3 protein (arrow).)
Related Product Information for anti-MYLK3 antibody
Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).
Product Categories/Family for anti-MYLK3 antibody
References
Al-Sadi, R., et al. J. Immunol. 180(8):5653-5661(2008). Chan, J.Y., et al. Circ. Res. 102(5):571-580(2008). Seguchi, O., et al. J. Clin. Invest. 117(10):2812-2824(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,723 Da
NCBI Official Full Name
myosin light chain kinase 3
NCBI Official Synonym Full Names
myosin light chain kinase 3
NCBI Official Symbol
MYLK3
NCBI Official Synonym Symbols
MLCK; MLCK2; caMLCK
NCBI Protein Information
myosin light chain kinase 3; MLC kinase; cardiac-MLCK; putative myosin light chain kinase 3; cardiac-MyBP-C associated Ca/CaM kinase; cardiac-MyBP-C-associated Ca/CaM kinase
UniProt Protein Name
Myosin light chain kinase 3
Protein Family
UniProt Gene Name
MYLK3
UniProt Synonym Gene Names
MLCK; Cardiac-MLCK
Uniprot Description
caMLCK: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: CAMK group; EC 2.7.11.18; Kinase, protein; MLCK family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 16q11.2
Cellular Component: actin cytoskeleton; cytoplasm; cytosol; intracellular
Molecular Function: ATP binding; calmodulin-dependent protein kinase activity; myosin light chain kinase activity
Biological Process: cardiac myofibril assembly; cellular response to interleukin-1; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; protein amino acid phosphorylation; regulation of vascular permeability during acute inflammatory response; sarcomere organization; sarcomerogenesis
Similar Products
Product Notes
The MYLK3 mylk3 (Catalog #AAA6323389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYLK3, ID (MYLK3, MLCK, Putative myosin light chain kinase 3, Cardiac-MyBP-C-associated Ca/CaM kinase) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYLK3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MYLK3 mylk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYLK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
