Western Blot (WB)
(Western blot analysis of lysate from mouse kidney tissue lysate, using MYLIP Antibody (Center). MBS9208904 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Rabbit anti-Human, mouse MYLIP Polyclonal Antibody | anti-MYLIP antibody
MYLIP Antibody (Center)
Western Blot (WB)
(Western blot analysis of lysate from mouse kidney tissue lysate, using MYLIP Antibody (Center). MBS9208904 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of lysate from mouse kidney tissue lysate, using MYLIP Antibody (Center). MBS9208904 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(MYLIP Antibody (Center T126) western blot analysis in MCF-7 cell line lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow))
Western Blot (WB)
(MYLIP Antibody (Center T126) western blot analysis in MCF-7 cell line lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow))
Western Blot (WB)
(MYLIP Antibody (Center T126) western blot analysis in mouse heart tissue lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow).)
Western Blot (WB)
(MYLIP Antibody (Center T126) western blot analysis in mouse heart tissue lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow).)
Immunohistochemistry (IHC)
(MYLIP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the MYLIP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MYLIP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the MYLIP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(MYLIP Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MYLIP Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Lindholm, D., et al. Cell. Mol. Life Sci. 66(21):3399-3402(2009)
Zelcer, N., et al. Science 325(5936):100-104(2009)
NCBI and Uniprot Product Information
NCBI Description
The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008]
Uniprot Description
MYLIP: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; Ubiquitin ligase; Motility/polarity/chemotaxis; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 6p22.3
Cellular Component: extrinsic to membrane; cytoskeleton; cytoplasm; plasma membrane; intracellular
Molecular Function: protein binding; zinc ion binding; cytoskeletal protein binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: nervous system development; cholesterol homeostasis; protein destabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; protein ubiquitination; regulation of low-density lipoprotein receptor catabolic process; cell motility
Research Articles on MYLIP
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Product Notes
The MYLIP mylip (Catalog #AAA9208904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 111-139. The MYLIP Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MYLIP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the MYLIP mylip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYLIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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