Rabbit anti-Human, Mouse MYLIP Polyclonal Antibody | anti-MYLIP antibody

MYLIP, ID (MYLIP, BZF1, IDOL, E3 ubiquitin-protein ligase MYLIP, Inducible degrader of the LDL-receptor, Myosin regulatory light chain interacting protein) (FITC)

Cat. Num. AAA6323377

• Gene Names: MYLIP; MIR; IDOL
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Peptide Immunoaffinity Chromatography.
• Synonyms: MYLIP; Polyclonal Antibody; ID (MYLIP; BZF1; IDOL; E3 ubiquitin-protein ligase MYLIP; Inducible degrader of the LDL-receptor; Myosin regulatory light chain interacting protein) (FITC); Myosin regulatory light chain interacting protein; anti-MYLIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Peptide Immunoaffinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MYLIP antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MYLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Central region of human MYLIP.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MYLIP Antibody (Center T126) western blot analysis in MCF-7 cell line lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow))

Western Blot (WB)

(MYLIP Antibody (Center T126) western blot analysis in mouse heart tissue lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow).)

Immunohistochemistry (IHC)

(MYLIP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the MYLIP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MYLIP Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MYLIP antibody

The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth.

Product Categories/Family for anti-MYLIP antibody

Antibodies; Ubiquitin Ligases

References

Chasman, D.I., et al. PLoS Genet. 5 (11), E1000730 (2009). Lindholm, D., et al. Cell. Mol. Life Sci. 66(21):3399-3402(2009). Zelcer, N., et al. Science 325(5936):100-104(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 38788243
• NCBI GeneID: 29116
• NCBI Accession #: NP_037394
• NCBI GenBank Nucleotide #: NM_013262
• UniProt Accession #: Q8WY64
• Molecular Weight: 50kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase MYLIP
• NCBI Official Synonym Full Names: myosin regulatory light chain interacting protein
• NCBI Official Symbol: MYLIP
• NCBI Official Synonym Symbols: MIR; IDOL
• NCBI Protein Information: E3 ubiquitin-protein ligase MYLIP
• UniProt Protein Name: E3 ubiquitin-protein ligase MYLIP
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: MYLIP
• UniProt Synonym Gene Names: BZF1; IDOL; Idol; MIR
• UniProt Entry Name: MYLIP_HUMAN

NCBI Description

The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008]

Uniprot Description

MYLIP: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Motility/polarity/chemotaxis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: extrinsic to membrane; cytoskeleton; cytoplasm; plasma membrane; intracellular

Molecular Function: protein binding; zinc ion binding; cytoskeletal protein binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: nervous system development; cholesterol homeostasis; protein destabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; protein ubiquitination; regulation of low-density lipoprotein receptor catabolic process; cell motility