Goat anti-Human MUNC18-2 Polyclonal Antibody | anti-Stxbp2 antibody

MUNC18-2 (Syntaxin-binding Protein 2, Syntaxin-BP2, STXBP2, Hunc18b, Unc18-2)

Cat. Num. AAA620026

• Gene Names: Stxbp2; Stxbp2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MUNC18-2; Polyclonal Antibody; MUNC18-2 (Syntaxin-binding Protein 2; Syntaxin-BP2; STXBP2; Hunc18b; Unc18-2); Anti -MUNC18-2 (Syntaxin-binding Protein 2; anti-Stxbp2 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MUNC18-2 (Syntaxin-BP2). Shows ~5% crossreactivity with recombinant human Syntaxin-BP1 and Syntaxin-BP3 in direct ELISA. Species Sequence Homology: Mouse - 95%.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

• Applicable Applications for anti-Stxbp2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well.; Western Blot: 1ug/ml
• Immunogen: Recombinant corresponding to aa81-223 of human MUNC18-2 expressed in E. coli (NP_001120868).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of Syntaxin-BP2 with MBS620026. PBL cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and incubated with 1ug/ml MBS620026.)

Related Product Information for anti-Stxbp2 antibody

MUNC18-2 (Syntaxin-binding protein 2; STXBP2, Unc18-2, Unc18B) is a 64-66kD member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins. It is widely expressed, particularly in hematopoietic cells, and is found both in cytoplasm, and associated with membranes, where it binds to STX1A, 2 and 3. Thus, it presumably participates in primary granule exocytosis by promoting granule fusion. Human STXBP2 is 593aa in length. It contains one SEC1 domain (aa28-576) with Trp28, Ser42 and Glu59 all playing a key role in syntaxin binding. There is one splice variant that shows a deletion of aa83-85. Over aa80-223, human and mouse STXBP2 share 95% amino acid sequence identity.

Product Categories/Family for anti-Stxbp2 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1022681
• NCBI GeneID: 81804
• UniProt Accession #: Q62753
• Molecular Weight: 66,696 Da
• NCBI Official Full Name: Munc18-2
• NCBI Official Synonym Full Names: syntaxin binding protein 2
• NCBI Official Symbol: Stxbp2
• NCBI Official Synonym Symbols: Stxbp2
• NCBI Protein Information: syntaxin-binding protein 2; unc-18B; unc18-2; munc18-2; unc-18 homolog 2; protein unc-18 homolog 2; protein unc-18 homolog B; syntaxin binding protein Munc18-2
• UniProt Protein Name: Syntaxin-binding protein 2
• Protein Family: Syntaxin-binding protein
• UniProt Gene Name: Stxbp2
• UniProt Synonym Gene Names: Unc18b
• UniProt Entry Name: STXB2_RAT

NCBI Description

syntaxin binding protein; important for synaptic vesicle exoctyosis; may be a component of the SNARE complex; may be involved with pancreatic beta-cell exocytosis [RGD]

Uniprot Description

STXBP2: Involved in intracellular vesicle trafficking and vesicle fusion with membranes. Contributes to the granule exocytosis machinery through interaction with soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that regulate membrane fusion. Regulates cytotoxic granule exocytosis in natural killer (NK) cells (By similarity)

Cellular Component: SNARE complex; azurophil granule; specific granule; protein complex; cell; lamellipodium; apical plasma membrane; cytoplasm; plasma membrane; cytosol; secretory granule

Molecular Function: protein binding; syntaxin binding; syntaxin-1 binding; syntaxin-3 binding

Biological Process: regulation of mast cell degranulation; protein transport; synaptic vesicle exocytosis; exocytosis; leukocyte mediated cytotoxicity; neutrophil degranulation; response to acidity; vesicle docking during exocytosis