Rabbit anti-Human MUC20 Polyclonal Antibody | anti-MUC20 antibody

MUC20, CT (MUC20, KIAA1359, Mucin-20) (Azide free) (HRP)

Cat. Num. AAA6322938

• Gene Names: MUC20; MUC-20
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MUC20; Polyclonal Antibody; CT (MUC20; KIAA1359; Mucin-20) (Azide free) (HRP); Mucin-20; anti-MUC20 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MUC20.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MUC20 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 654-684 in the C-terminal region of human MUC20.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in WiDr cell line lysates (35ug/lane) usingMBS6006805. This demonstrates that MBS6006805 detected the MUC20 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in human lung tissue lysate (20ug) using MBS6006805 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 72kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-MUC20 antibody

Antibodies; Mucin

References

1. Li, G., Am. J. Nephrol. 26 (1), 43-49 (2006). 2. Suzuki, Y., Genome Res. 14 (9), 1711-1718 (2004). 3. Higuchi, T., Mol. Cell. Biol. 24 (17), 7456-7468 (2004). 4. Higuchi, T., J. Biol. Chem. 279 (3), 1968-1979 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 317373415
• NCBI GeneID: 200958
• UniProt Accession #: Q8N307; Q86ST8
• Molecular Weight: 538
• NCBI Official Full Name: Mucin-20
• NCBI Official Synonym Full Names: mucin 20, cell surface associated
• NCBI Official Symbol: MUC20
• NCBI Official Synonym Symbols: MUC-20
• NCBI Protein Information: mucin-20; transmembrane mucin MUC20S
• UniProt Protein Name: Mucin-20
• Protein Family: Mucin
• UniProt Gene Name: MUC20
• UniProt Synonym Gene Names: KIAA1359; MUC-20
• UniProt Entry Name: MUC20_HUMAN

NCBI Description

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014]

Uniprot Description

MUC20: May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: Golgi lumen; microvillus membrane; apical plasma membrane; basal plasma membrane; extracellular region

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; activation of MAPK activity; hepatocyte growth factor receptor signaling pathway; post-translational protein modification; protein homooligomerization