Western Blot (WB)
(Western Blot analysis in WiDr cell line lysates (35ug/lane) using MBS6006805. This demonstrates that MBS6006805 detected the MUC20 protein (arrow).)
Rabbit anti-Human MUC20 Polyclonal Antibody | anti-MUC20 antibody
MUC20, CT (MUC20, KIAA1359, Mucin-20)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western Blot analysis in WiDr cell line lysates (35ug/lane) using MBS6006805. This demonstrates that MBS6006805 detected the MUC20 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in WiDr cell line lysates (35ug/lane) using MBS6006805. This demonstrates that MBS6006805 detected the MUC20 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in human lung tissue lysate (20ug) using MBS6006805 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 72kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in human lung tissue lysate (20ug) using MBS6006805 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 72kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS6006805 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006805 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS6006805 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006805 for immunohistochemistry.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis with WiDr cell using MBS6006805 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis with WiDr cell using MBS6006805 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The shorter isoform expressed by this gene is localized to the plasma membrane, whereas the longer isoform might be secreted. The C terminus of this protein associates with the multifunctional docking site of the met proto-oncogene and suppresses activation of some downstream met signaling cascades. The protein features a tandem repeat domain that varies between 2 and 6 copies in most individuals. The allele represented in the human reference assembly contains 12 copies. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Uniprot Description
MUC20: May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 3q29
Cellular Component: microvillus membrane; Golgi lumen; apical plasma membrane; basal plasma membrane; extracellular region
Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; activation of MAPK activity; hepatocyte growth factor receptor signaling pathway; post-translational protein modification; protein homooligomerization
Research Articles on MUC20
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Product Notes
The MUC20 muc20 (Catalog #AAA6006805) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MUC20, CT (MUC20, KIAA1359, Mucin-20) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MUC20 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Immunofluorescence: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the MUC20 muc20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUC20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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