Testing Data
Rabbit Mu-Calpain Polyclonal Antibody | anti-Capn1 antibody
Anti-Mu-Calpain (E.C. 3.4.22.17, Calpain-I, Protein kinase-C Activating Factor), Domain-I Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Mu-Calpain; Polyclonal Antibody; Anti-Mu-Calpain (E.C. 3.4.22.17; Calpain-I; Protein kinase-C Activating Factor); Domain-I Antibody; Calpain-1; anti-Capn1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody recognizes Domain I of the large subunit of Calpain 1. The antibody does not recognize amino-processed Calpain 1, and can be used to discriminate between latent and amino-processed forms. Also ab28258 does not recognize the other calpain family members (M calpain, calpain 94, ncl 2, ncl 3, etc.).
Purity/Purification
Immunogen Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
713
Applicable Applications for anti-Capn1 antibody
Western Blot (WB)
Application Notes
Both M-calpain and m-calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain-94, ncl-2, ncl-3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain-4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV. RP2Calpain-1 recognizes latent, but not amino-processed Mu-calpain; the latent large subunit is 80 Kd, and the amino terminal truncation at activation yields an approximately 58 Kd form. Cleavage of the carboxyterminal region generates smaller forms of Mu-Calpain, but it is not clear if these forms are proteolytically active. RP2Calpain-1 Anti-Mu-Calpain works well for Western Blotting applications, and when used against the reduced protein identifies bands at 80 Kd, 68 Kd, and a series of further cleaved forms. RP2Calpain-1 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. Most cell types produce Mu-Calpain; since it is a cellular protein, cell lysate works well for Western blots. Rat, mouse and human Mu-Calpain are detected with RP2Calpain-1. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.
Immunogen
Synthetic peptide based on Domain I of the large subunit of human calpain 1.
Cellular Localization
Cytoplasmic. Cell Membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-Capn1 antibody
Description: The antibody is a Rabbit antibody to the cysteine protease, Mu-calpain, made with a synthetic peptide based on Domain-I of the large subunit, using the human sequence. The antibody binds to Mu-calpain, but does not cross react with the other calpain family members (M-calpain, calpain-94, ncl-2, ncl-3, etc.). RP2Calpain-1 reacts to the-propeptide domain,-(Domain-I) of Calpain-1, immediately aminoterminal from the activation cleavage site. The antibody does not recognize amino-processed Calpain-1, and can be used to discriminate between latent and amino-processed forms. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
Relevance: Mu Calpain, also known as Calpain I, and m calpain, also known as Calpain II, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. The calpains have papain like activity, thus the-pain nomenclature. Both m calpain and mu calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach specific, and muscle specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit.
Relevance: Mu Calpain, also known as Calpain I, and m calpain, also known as Calpain II, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. The calpains have papain like activity, thus the-pain nomenclature. Both m calpain and mu calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach specific, and muscle specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit.
NCBI and Uniprot Product Information
NCBI Description
calcium-dependent protease that may be involved in experimental allergic encephalomyelitis (EAE) [RGD, Feb 2006]
Research Articles on Capn1
Similar Products
Product Notes
The Capn1 (Catalog #AAA4157385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mu-Calpain (E.C. 3.4.22.17, Calpain-I, Protein kinase-C Activating Factor), Domain-I Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mu-Calpain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Both M-calpain and m-calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain-94, ncl-2, ncl-3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain-4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV. RP2Calpain-1 recognizes latent, but not amino-processed Mu-calpain; the latent large subunit is 80 Kd, and the amino terminal truncation at activation yields an approximately 58 Kd form. Cleavage of the carboxyterminal region generates smaller forms of Mu-Calpain, but it is not clear if these forms are proteolytically active. RP2Calpain-1 Anti-Mu-Calpain works well for Western Blotting applications, and when used against the reduced protein identifies bands at 80 Kd, 68 Kd, and a series of further cleaved forms. RP2Calpain-1 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. Most cell types produce Mu-Calpain; since it is a cellular protein, cell lysate works well for Western blots. Rat, mouse and human Mu-Calpain are detected with RP2Calpain-1. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Researchers should empirically determine the suitability of the Capn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mu-Calpain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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