Testing Data
Rabbit M-Calpain Polyclonal Antibody
Anti-M-Calpain (E.C. 3.4.22.17, Calpain-II, Protein kinase-C Activating Factor), Domain IV Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
M-Calpain; Polyclonal Antibody; Anti-M-Calpain (E.C. 3.4.22.17; Calpain-II; Protein kinase-C Activating Factor); Domain IV Antibody; Calpain-2; anti-M-Calpain antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody recognises the Carboxyterminal end of the large subunit of calpain 2, both in its latent and aminoprocessed (activated) forms. It does not recognise the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.).
Purity/Purification
Immunogen Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
698
Applicable Applications for anti-M-Calpain antibody
Western Blot (WB)
Application Notes
M-Calpain, also known as Calpain-II, is an intracellular, calcium-dependent cysteine protease. M-calpain has a Millimolar sensitivity (thus the M-) as compared to the micromolar calcium sensitivity of m-calpain. The calpains have papain-like activity, thus the pain nomenclature. Both M-calpain and m-calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain-94, ncl-2, ncl-3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain-4), and a large variable subunit (although it is not clear if all calpains contain a small subunit). Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV. RP3-Calpain2 recognizes latent and active M-calpain; the latent large subunit is 80 Kd, and the amino terminal truncation at activation yields an approximately 58 Kd form. A cascade of smaller forms can be seen with further activation. RP3Calpain2 Anti-M-Calpain works well for Western Blotting applications, and when used against the reduced protein identifies bands at 80 Kd, 58 Kd, and a series of further cleaved active forms. RP3Calpain2 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. Most cell types produce M-Calpain; since it is a cellular protein, cell lysate works well for Western blots. Rat, mouse and human M-Calpain are detected with RP3Calpain-2. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.
Immunogen
Synthetic peptide based on the Carboxyterminal end of the large subunit of human calpain 2.
Cellular Localization
Cytoplasmic. Cell Membrane. Translocates to the plasma membrane upon Ca2+ binding.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-M-Calpain antibody
Description: The antibody is a Rabbit antibody to the cysteine protease, M-calpain, made with a synthetic peptide based on the Carboxyterminal end of the large subunit, using the human sequence. The antibody binds to M-calpain, but does not cross react with the other calpain family members (m-calpain, calpain-94, ncl-2, ncl-3, etc.). RP3Calpain-2 reacts to the carboxyterminal end of Calpain-2, and detects both latent and aminoprocessed (activated) Calpain-2. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
Relevance: Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Relevance: Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
m-calpain
Similar Products
Product Notes
The M-Calpain (Catalog #AAA4157390) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-M-Calpain (E.C. 3.4.22.17, Calpain-II, Protein kinase-C Activating Factor), Domain IV Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's M-Calpain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). M-Calpain, also known as Calpain-II, is an intracellular, calcium-dependent cysteine protease. M-calpain has a Millimolar sensitivity (thus the M-) as compared to the micromolar calcium sensitivity of m-calpain. The calpains have papain-like activity, thus the pain nomenclature. Both M-calpain and m-calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain-94, ncl-2, ncl-3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain-4), and a large variable subunit (although it is not clear if all calpains contain a small subunit). Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV. RP3-Calpain2 recognizes latent and active M-calpain; the latent large subunit is 80 Kd, and the amino terminal truncation at activation yields an approximately 58 Kd form. A cascade of smaller forms can be seen with further activation. RP3Calpain2 Anti-M-Calpain works well for Western Blotting applications, and when used against the reduced protein identifies bands at 80 Kd, 58 Kd, and a series of further cleaved active forms. RP3Calpain2 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. Most cell types produce M-Calpain; since it is a cellular protein, cell lysate works well for Western blots. Rat, mouse and human M-Calpain are detected with RP3Calpain-2. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Researchers should empirically determine the suitability of the M-Calpain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "M-Calpain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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