Western Blot (WB)
(Anti-MTSS1 rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:50 kDaObserved band size:56 kDa)
Rabbit anti-Human MTSS1 Polyclonal Antibody | anti-MTSS1 antibody
Anti-MTSS1 Antibody, Rabbit Polyclonal
Gene Names
MTSS1; MIM; MIMA; MIMB
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
MTSS1; Polyclonal Antibody; Anti-MTSS1 Antibody; Rabbit Polyclonal; MTSS1 Antibody; Rabbit PAb; Antigen Affinity Purified; metastasis suppressor 1; Anti-MIM Antibody; Anti-MIMA Antibody; Anti-MIMB Antibody; anti-MTSS1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MTSS1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MTSS1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
ELISA: 1:25,000-1:50,000
Immunogen
Recombinant Human MTSS1 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human MTSS1 (rh MTSS1). MTSS1 specific IgG was purified by Human MTSS1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-MTSS1 rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:50 kDaObserved band size:56 kDa)
Western Blot (WB)
(Anti-MTSS1 rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:50 kDaObserved band size:56 kDa)
Related Product Information for anti-MTSS1 antibody
MTSS1 (Metastasis suppressor 1), also known as Missing in metastasis (MIM), is a tissue-specific regulator of plasma membrane dynamics. MTSS1 is well described for its function as a metastasis suppressor gene and is expressed in a variety of tissues. MTSS1 might be involved in shaping neuronal membranes in vivo. MTSS1 deforms phosphoinositide-rich membranes through its I-BAR domain and interacts with actin monomers through its WH2 domain. MTSS1/MIM was first identified as a metastasis suppressor missing in metastatic bladder carcinoma cell lines. MTSS1 is a prognostic indicator of disease-free survival in breast cancer patients and demonstrates the ability to play a role in governing the metastatic nature of breast cancer cells. MTSS1 may serve as a useful biomarker for the prediction of the outcome of gastric cancer. The down-regulation of MTSS1 that may be caused by DNA methylation was also observed in many other types of cancer. Recent work proposed that MIM also potentiates Sonic hedgehog (Shh)-induced gene expression. MTSS1 is a multiple functional molecular and has an important role in development, carcinogenesis, and metastasis.
References
Hayn-Leichsenring G, et al. (2011) Cellular distribution of metastasis suppressor 1 and the shape of cell bodies are temporarily altered in Engrailed-2 overexpressing cerebellar Purkinje cells. Neuroscience. 189: 68-78.Xie F, et al. (2011) MTSS1: a multifunctional protein and its role in cancer invasion and metastasis. Front Biosci (Schol Ed). 3: 621-31.Saarikangas J, et al. (2011) Missing-in-metastasis MIM/MTSS1 promotes actin assembly at intercellular junctions and is required for integrity of kidney epithelia. J Cell Sci. 2124(Pt 8): 1245-55.Parr C, et al. (2009) Metastasis suppressor 1 (MTSS1) demonstrates prognostic value and anti-metastatic properties in breast cancer. Eur J Cancer. 45(9): 1673-83.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Mol_Mass: 71.8 kDa
AP_Mol_Mass: 66 kDa
AP_Mol_Mass: 66 kDa
NCBI Official Full Name
metastasis suppressor 1, isoform CRA_e
NCBI Official Synonym Full Names
MTSS1, I-BAR domain containing
NCBI Official Symbol
MTSS1
NCBI Official Synonym Symbols
MIM; MIMA; MIMB
NCBI Protein Information
metastasis suppressor protein 1
Protein Family
Research Articles on MTSS1
1. Down-regulation of MTSS1 expression is associated with lymph node metastasis in Pancreatic Cancer.
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Product Notes
The MTSS1 (Catalog #AAA8106699) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MTSS1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MTSS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000. Researchers should empirically determine the suitability of the MTSS1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTSS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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