Western Blot (WB)
(Western blot analysis of extracts of HepG2 cells, using MTHFD1L antibody.)
Rabbit MTHFD1L Polyclonal Antibody | anti-MTHFD1L antibody
MTHFD1L Antibody
Gene Names
MTHFD1L; FTHFSDC1; MTC1THFS; dJ292B18.2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MTHFD1L; Polyclonal Antibody; MTHFD1L Antibody; 10-formyl-THF synthetase; C1TM_HUMAN; Formyltetrahydrofolate synthetase; formyltetrahydrofolate synthetase domain containing 1; FTHFSDC1; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like; mitochondrial; Monofunctional C1-tetrahydrofolate synthase; MTC1THFS; RP1-292B18.2; anti-MTHFD1L antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MTHFD1L antibody detects endogenous levels of MTHFD1L.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
979
Applicable Applications for anti-MTHFD1L antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human MTHFD1L.
Subcellular Location
Mitochondrion.
Tissue Specificity
Detected in most tissues, highest expression found in placenta, thymus and brain. Low expression is found in liver and skeletal muscle. Up-regulated in colon adenocarcinoma.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (80%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of HepG2 cells, using MTHFD1L antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HepG2 cells, using MTHFD1L antibody.)
Immunohistochemistry (IHC)
(MBS9603653 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603653 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-MTHFD1L antibody
Function: May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.
Subunit Structure: Homodimer.
Similarity: This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain.In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.
Subunit Structure: Homodimer.
Similarity: This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain.In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 106 kDa
Predicted: 106 kDa
Predicted: 106 kDa
NCBI Official Full Name
monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 1
NCBI Official Synonym Full Names
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like
NCBI Official Symbol
MTHFD1L
NCBI Official Synonym Symbols
FTHFSDC1; MTC1THFS; dJ292B18.2
NCBI Protein Information
monofunctional C1-tetrahydrofolate synthase, mitochondrial
UniProt Protein Name
Monofunctional C1-tetrahydrofolate synthase, mitochondrial
Protein Family
UniProt Gene Name
MTHFD1L
UniProt Synonym Gene Names
FTHFSDC1
NCBI Description
The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]
Uniprot Description
May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.
Research Articles on MTHFD1L
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Product Notes
The MTHFD1L mthfd1l (Catalog #AAA9603653) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MTHFD1L Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MTHFD1L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the MTHFD1L mthfd1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTHFD1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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