Rabbit anti-Human SEMG1 Polyclonal Antibody | anti-SEMG1 antibody

SEMG1, NT (SEMG1, SEMG, Semenogelin-1, Semenogelin I, Alpha-inhibin-92, Alpha-inhibin-31, Seminal basic protein) (Biotin)

Cat. Num. AAA6346044

• Gene Names: SEMG1; SGI; SEMG; CT103; dJ172H20.2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SEMG1; Polyclonal Antibody; NT (SEMG1; SEMG; Semenogelin-1; Semenogelin I; Alpha-inhibin-92; Alpha-inhibin-31; Seminal basic protein) (Biotin); Seminal basic protein; anti-SEMG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SEMG1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SEMG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-121 amino acids from the N-terminal region of human SEMG1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SEMG1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SEMG1 antibody detected the SEMG1 protein (arrow).)

Related Product Information for anti-SEMG1 antibody

The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely.

Product Categories/Family for anti-SEMG1 antibody

Antibodies; Spermatogenesis; Testis Proteins

References

Mitra, A., et al. Biol. Reprod. 82(3):489-496(2010). Yoshida, K., et al. Cell Motil. Cytoskeleton 66(2):99-108(2009). Ahmed, S.U., et al. Cytotherapy 11(2):238-244(2009). Edstrom, A.M., et al. J. Immunol. 181(5):3413-3421(2008). Emami, N., et al. J. Biol. Chem. 283(28):19561-19569(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4506883
• NCBI GeneID: 6406
• NCBI Accession #: NP_002998.1
• NCBI GenBank Nucleotide #: NM_003007.3
• UniProt Accession #: P04279; Q53ZV0; Q53ZV1; Q53ZV2; Q6X4I9; Q6Y809; Q6Y822; Q6Y823; Q86U64; Q96QM3
• Molecular Weight: 45,322 Da
• NCBI Official Full Name: semenogelin-1 preproprotein
• NCBI Official Synonym Full Names: semenogelin I
• NCBI Official Symbol: SEMG1
• NCBI Official Synonym Symbols: SGI; SEMG; CT103; dJ172H20.2
• NCBI Protein Information: semenogelin-1; SgI-29; cancer/testis antigen 103; semen coagulating protein
• UniProt Protein Name: Semenogelin-1
• Protein Family: Semenogelin
• UniProt Gene Name: SEMG1
• UniProt Synonym Gene Names: SEMG; SGI
• UniProt Entry Name: SEMG1_HUMAN

NCBI Description

The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. The antimicrobial peptide SgI-29 is an antimicrobial peptide with antibacterial activity. [provided by RefSeq, Nov 2014]

Uniprot Description

SEMG1: Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA. Belongs to the semenogelin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 20q12-q13.2

Cellular Component: extracellular space; extracellular region; nucleus; secretory granule

Molecular Function: protein binding; structural molecule activity

Biological Process: insemination