Rabbit anti-Human MTHFD1L Polyclonal Antibody | anti-MTHFD1L antibody

MTHFD1L Antibody

Cat. Num. AAA7046535

• Gene Names: MTHFD1L; FTHFSDC1; MTC1THFS; dJ292B18.2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: MTHFD1L; Polyclonal Antibody; MTHFD1L Antibody; Monofunctional C1-tetrahydrofolate synthase; mitochondrial; Formyltetrahydrofolate synthetase; FTHFSDC1; anti-MTHFD1L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 979

• Applicable Applications for anti-MTHFD1L antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human Monofunctional C1-tetrahydrofolate synthase, mitochondrial protein (C-220AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: MTHFD1L antibody at 3.68ug/mlLane 1: Colo320 whole cell lysateLane 2: HepG-2 whole cell lysateLane 3: Hela whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 106,30 kDaObserved band size: 106 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7046535 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7046535 at dilution 1:100)

Related Product Information for anti-MTHFD1L antibody

May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.

NCBI and Uniprot Product Information

• NCBI GI #: 337756501
• NCBI GeneID: 25902
• NCBI Accession #: NP_001229696.1
• NCBI GenBank Nucleotide #: NM_001242767.1
• UniProt Accession #: Q6UB35; Q2TBF3; Q8WVW0; Q96HG8; Q9H789; Q9UFU8
• Molecular Weight: 29,804 Da
• NCBI Official Full Name: monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
• NCBI Official Symbol: MTHFD1L
• NCBI Official Synonym Symbols: FTHFSDC1; MTC1THFS; dJ292B18.2
• NCBI Protein Information: monofunctional C1-tetrahydrofolate synthase, mitochondrial
• UniProt Protein Name: Monofunctional C1-tetrahydrofolate synthase, mitochondrial
• Protein Family: Monofunctional C1-tetrahydrofolate synthase
• UniProt Gene Name: MTHFD1L
• UniProt Synonym Gene Names: FTHFSDC1
• UniProt Entry Name: C1TM_HUMAN

NCBI Description

The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

MTHFD1L: May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 6.3.4.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Cofactor and Vitamin Metabolism - one carbon pool by folate; Ligase

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: cytosol; membrane; mitochondrial matrix; mitochondrion

Molecular Function: ATP binding; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity; protein homodimerization activity

Biological Process: embryonic neurocranium morphogenesis; embryonic viscerocranium morphogenesis; folic acid and derivative metabolic process; folic acid metabolic process; formate metabolic process; neural tube closure; one-carbon compound metabolic process; tetrahydrofolate metabolic process