Rabbit Vasodilator Stimulated Phosphoprotein Polyclonal Antibody | anti-VASP antibody

Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser157) (VASP)

Cat. Num. AAA612013

• Gene Names: VASP; VASP
• Reactivity: Guinea Pig, Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: Vasodilator Stimulated Phosphoprotein; Polyclonal Antibody; phosphorylated (Ser157) (VASP); Anti -Vasodilator Stimulated Phosphoprotein; anti-VASP antibody

• Host: Rabbit
• Reactivity: Guinea Pig, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human VASP phosphorylated at Ser157. May crossreact with the phosphorylated VASP homologue Mena. Species crossreactivity: mouse, rat, monkey and guinea pig.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

• Applicable Applications for anti-VASP antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry: (Paraffin) 1:50
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser157 of human VASP.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from A431 cells stimulated with forskolin for indicated times, using Phospho-VASP (Ser157) Antibody (upper) and VASP Antibody #3112 (lower).)

Western Blot (WB)

(Western blot analysis of extracts from untreated and 8-Br-cGMP, 8-Br-cAMP or forskolin treated NIH/3T3 and C6 cells, using Phospho-VASP (Ser157) Antibody.)

Testing Data

Testing Data

Related Product Information for anti-VASP antibody

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP-and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239 and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful tool to monitor PKG activation and signaling (6,7).

Product Categories/Family for anti-VASP antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4507869
• NCBI GeneID: 7408
• NCBI Accession #: NP_003361.1
• NCBI GenBank Nucleotide #: NM_003370.3
• UniProt Accession #: P50552; Q6PIZ1; Q93035; B2RBT9
• Molecular Weight: 39,830 Da
• NCBI Official Full Name: vasodilator-stimulated phosphoprotein
• NCBI Official Synonym Full Names: vasodilator-stimulated phosphoprotein
• NCBI Official Symbol: VASP
• NCBI Official Synonym Symbols: VASP
• NCBI Protein Information: vasodilator-stimulated phosphoprotein
• UniProt Protein Name: Vasodilator-stimulated phosphoprotein
• Protein Family: Vasodilator-stimulated phosphoprotein
• UniProt Gene Name: VASP
• UniProt Entry Name: VASP_HUMAN

NCBI Description

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq]

Uniprot Description

VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: filopodium membrane; focal adhesion; tight junction; cytoplasm; plasma membrane; cytosol; actin cytoskeleton

Molecular Function: protein binding; actin binding; SH3 domain binding; profilin binding

Biological Process: axon guidance; positive regulation of actin filament polymerization; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway; protein homotetramerization