Rabbit anti-Human Mst3 Polyclonal Antibody | anti-Mst3 antibody

Mst3, CT (Mammalian STE20-like Protein Kinase 3, MST-3, MST3B, Serine/threonine Kinase 24 (Ste20, Yeast Homolog), STE20 Homolog (yeast), STE20-like Kinase MST3, STK3, STK24) (Azide free) (HRP)

Cat. Num. AAA6488647

• Gene Names: STK24; MST3; STK3; MST3B; STE20
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Mst3; Polyclonal Antibody; CT (Mammalian STE20-like Protein Kinase 3; MST-3; MST3B; Serine/threonine Kinase 24 (Ste20; Yeast Homolog); STE20 Homolog (yeast); STE20-like Kinase MST3; STK3; STK24) (Azide free) (HRP); EC=2.7.11.1; Serine/threonine-protein Kinase 24; anti-Mst3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MST3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Mst3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MST3 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MST3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). MST3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Mst3 antibody

Antibodies; Protein Kinases

References

1. Huang, C.Y., et al., J. Biol. Chem. 277(37):34367-34374 (2002). 2. Christian, S.L., et al., Genomics 79(5):635-656 (2002). 3. Zhou, T.H., et al., J. Biol. Chem. 275(4):2513-2519 (2000). 4. Schinkmann, K., et al., J. Biol. Chem. 272(45):28695-28703 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 110349738
• NCBI GeneID: 8428
• NCBI Accession #: NP_001027467.2
• NCBI GenBank Nucleotide #: NM_001032296.2
• UniProt Accession #: Q9Y6E0; O14840; Q5JV92
• Molecular Weight: 49,308 Da
• NCBI Official Full Name: serine/threonine-protein kinase 24 isoform b
• NCBI Official Synonym Full Names: serine/threonine kinase 24
• NCBI Official Symbol: STK24
• NCBI Official Synonym Symbols: MST3; STK3; MST3B; STE20
• NCBI Protein Information: serine/threonine-protein kinase 24; STE20-like kinase 3; STE20-like kinase MST3; sterile 20-like kinase 3; mammalian STE20-like protein kinase 3; serine/threonine kinase 24 (STE20 homolog, yeast)
• UniProt Protein Name: Serine/threonine-protein kinase 24
• UniProt Gene Name: STK24
• UniProt Synonym Gene Names: MST3; STK3; MST-3; MST3/N; MST3/C
• UniProt Entry Name: STK24_HUMAN

NCBI Description

The yeast 'Sterile 20' gene (STE20) functions upstream of the mitogen-activated protein kinase (MAPK) cascade. In mammals, protein kinases related to STE20 can be divided into 2 subfamilies based on their structure and regulation. Members of the PAK subfamily (see PAK3; MIM 300142) contain a C-terminal catalytic domain and an N-terminal regulatory domain that has a CDC42 (MIM 116952)-binding domain. In contrast, members of the GCK subfamily (see MAP4K2; MIM 603166), also called the Sps1 subfamily, have an N-terminal catalytic domain and a C-terminal regulatory domain without a CDC42-binding domain. STK24 belongs to the GCK subfamily of STE20-like kinases (Zhou et al., 2000 [PubMed 10644707]).[supplied by OMIM, Mar 2008]

Uniprot Description

MST3: a protein kinase of the STE20 family. Activated by caspase-mediated cleavage

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; YSK subfamily

Chromosomal Location of Human Ortholog: 13q31.2-q32.3

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: regulation of axon regeneration; response to hydrogen peroxide; apoptosis; protein amino acid autophosphorylation; signal transduction; negative regulation of cell migration; protein amino acid phosphorylation; induction of apoptosis by oxidative stress; cell structure disassembly during apoptosis