Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using HDAC4/HDAC5/HDAC9 antibody)
Rabbit HDAC4+5+9 Polyclonal Antibody | anti-HDAC4 antibody
HDAC4+5+9 antibody
IHC: 1:50-1:100
WB: 1:500-1:1000
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using HDAC4/HDAC5/HDAC9 antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using HDAC4/HDAC5/HDAC9 antibody)
Western Blot (WB)
(Western Blot analysis of extracts from 293 and Hela cells using HDAC4/HDAC5/HDAC9 antibody)
Western Blot (WB)
(Western Blot analysis of extracts from 293 and Hela cells using HDAC4/HDAC5/HDAC9 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC4/HDAC5/HDAC9 antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC4/HDAC5/HDAC9 antibody (left) or the same antibody preincubated with with blocking peptide (right))
NCBI and Uniprot Product Information
NCBI Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]
Uniprot Description
HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
Protein type: Deacetylase; Hydrolase; EC 3.5.1.98; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: nucleoplasm; transcriptional repressor complex; histone deacetylase complex; cytoplasm; actomyosin; neuromuscular junction; Z disc; cytosol; nucleus; A band
Molecular Function: potassium ion binding; transcription activator binding; zinc ion binding; histone deacetylase binding; protein deacetylase activity; protein kinase binding; transcription factor binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity
Biological Process: cardiac muscle hypertrophy; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; response to denervation involved in regulation of muscle adaptation; regulation of gene expression, epigenetic; negative regulation of cell proliferation; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; negative regulation of glycolysis; inflammatory response; skeletal development; response to drug; nervous system development; regulation of skeletal muscle fiber development; B cell activation; transcription, DNA-dependent; negative regulation of transcription factor activity; histone deacetylation; osteoblast development; positive regulation of protein sumoylation; chromatin remodeling; B cell differentiation; regulation of protein binding; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent
Research Articles on HDAC4
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Product Notes
The HDAC4 hdac4 (Catalog #AAA837577) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HDAC4+5+9 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the HDAC4 hdac4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC4+5+9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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