Typical Testing Data/Standard Curve (for reference only)
Mouse Thrombospondin 2 (THBS2) CLIA Kit | THBS2 clia kit
Mouse Thrombospondin 2 (THBS2) CLIA Kit
Gene Names
Thbs2; TSP2; Thbs-2
Reactivity
Mouse
Applications
Chemiluminescence Immunoassay (CLIA)
Synonyms
Thrombospondin 2 (THBS2); Mouse Thrombospondin 2 (THBS2) CLIA Kit; TSP2; THBS2 clia kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of THBS2. No significant cross-reactivity or interference between THBS2 and analogues was observed.
Applicable Applications for THBS2 clia kit
Chemiluminescence Immunoassay (CLIA)
Application Notes
Chemiluminescent Immunoassay for Antigen Detection.
Samples
Mouse plasma, tissue homogenates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.112ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level THBS2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level THBS2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for THBS2 clia kit
Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of THBS2 in mouse plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to THBS2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to THBS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the THBS2 level in the sample or standard.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to THBS2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to THBS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the THBS2 level in the sample or standard.
Product Categories/Family for THBS2 clia kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
129,882 Da
NCBI Official Full Name
thrombospondin-2
NCBI Official Synonym Full Names
thrombospondin 2
NCBI Official Symbol
Thbs2
NCBI Official Synonym Symbols
TSP2; Thbs-2
NCBI Protein Information
thrombospondin-2
UniProt Protein Name
Thrombospondin-2
Protein Family
UniProt Gene Name
Thbs2
UniProt Synonym Gene Names
Tsp2
UniProt Entry Name
TSP2_MOUSE
Uniprot Description
THBS2: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. Genetic variations in THBS2 may be a cause of susceptibility to intervertebral disk disease (IDD); also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. Belongs to the thrombospondin family.
Protein type: Motility/polarity/chemotaxis
Cellular Component: basement membrane; extracellular matrix; extracellular region; proteinaceous extracellular matrix
Molecular Function: calcium ion binding; heparin binding; protein binding
Biological Process: cell adhesion; negative regulation of angiogenesis; positive regulation of synaptogenesis
Research Articles on THBS2
Similar Products
Product Notes
The Mouse THBS2 thbs2 (Catalog #AAA2124096) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2124096 CLIA Kit recognizes Mouse THBS2. AAA Biotech's Thrombospondin 2 (THBS2) can be used in a range of immunoassay formats including, but not limited to, Chemiluminescence Immunoassay (CLIA). Chemiluminescent Immunoassay for Antigen Detection. Researchers should empirically determine the suitability of the THBS2 thbs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thrombospondin 2 (THBS2), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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