Rabbit MRPL28 Polyclonal Antibody | anti-MRPL28 antibody

MRPL28 Polyclonal Antibody

Cat. Num. AAA2528331

• Gene Names: MRPL28; p15; MAAT1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: MRPL28; Polyclonal Antibody; MRPL28 Polyclonal Antibody; p15; MAAT1; anti-MRPL28 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 2.7mg/mL (varies by lot)
• Sequence Length: 128

• Applicable Applications for anti-MRPL28 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:5000; IHC: 1:100-1:300
• Immunogen: Recombinant protein of human MRPL28
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of 293T cell and Human testis tissue, A375 cell and Human normal kidney tissue using MRPL28 Polyclonal Antibody at dilution of 1:600)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer using MRPL28 Polyclonal Antibody at dilution of 1:45)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human cervical cancer using MRPL28 Polyclonal Antibody at dilution of 1:45)

Related Product Information for anti-MRPL28 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.

NCBI and Uniprot Product Information

• NCBI GI #: 49456461
• NCBI GeneID: 10573
• Molecular Weight: 30,157 Da
• NCBI Official Full Name: MRPL28, partial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L28
• NCBI Official Symbol: MRPL28
• NCBI Official Synonym Symbols: p15; MAAT1
• NCBI Protein Information: 39S ribosomal protein L28, mitochondrial; L28mt; MRP-L28; melanoma antigen p15; melanoma-associated antigen recognised by cytotoxic T lymphocytes; melanoma-associated antigen recognized by T lymphocytes; melanoma-associated antigen recognized by T-lymphocytes
• UniProt Protein Name: 39S ribosomal protein L28, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL28
• UniProt Synonym Gene Names: MAAT1; L28mt; MRP-L28
• UniProt Entry Name: RM28_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL28: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation; Mitochondrial

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial ribosome

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mitochondrial translation; translation; organelle organization and biogenesis