Human RSPO1 ELISA Kit | RSPO1 elisa kit

Human RSPO1 ELISA Kit

Cat. Num. AAA8248393

• Gene Names: RSPO1; RSPO; CRISTIN3
• Reactivity: Human
• Synonyms: RSPO1; Human RSPO1 ELISA Kit; R-spondin-1; Roof plate-specific spondin-1; hRspo1; RSPO1 elisa kit

• Reactivity: Human
• Specificity: The Human RSPO1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human RSPO1 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 263

• Samples: Cell culture supernates, serum, plasma
• Sensitivity: < 10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for RSPO1 elisa kit

Principle of the Assay: The MyBioSource' Human RSPO1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human RSPO1 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human RSPO1 coated on a 96-well plate. Standards and samples are pipetted into the wells and RSPO1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human RSPO1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RSPO1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: R-spondin-1 is a secreted protein that in humans is encoded by the Rspo1 gene. It is mapped to chromosome 1p34. This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy- induced adverse effects. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 84490388
• NCBI GeneID: 284654
• NCBI Accession #: NP_001033722.1
• NCBI GenBank Nucleotide #: NM_001038633.3
• UniProt Accession #: Q2MKA7; Q0H8S6; Q14C72; Q5T0F2; Q8N7L5; A2A420
• Molecular Weight: 21,775 Da
• NCBI Official Full Name: R-spondin-1 isoform 1
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: RSPO1
• NCBI Official Synonym Symbols: RSPO; CRISTIN3
• NCBI Protein Information: R-spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: RSPO1
• UniProt Synonym Gene Names: hRspo1
• UniProt Entry Name: RSPO1_HUMAN

NCBI Description

This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: extracellular region

Molecular Function: G-protein-coupled receptor binding; protein binding; receptor binding

Biological Process: positive regulation of protein amino acid phosphorylation; positive regulation of Wnt receptor signaling pathway; regulation of receptor internalization

Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal