Human Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) RTU ELISA Kit | ACAT1 rtu elisa kit

Human Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) ELISA Kit

Cat. Num. AAA4503437

• Gene Names: ACAT1; T2; MAT; ACAT; THIL
• Reactivity: Human
• Synonyms: Acetyl Coenzyme A Acetyltransferase 1 (ACAT1); Human Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) ELISA Kit; THIL; ACAT; T2; MAT; Acetoacetyl Coenzyme A Thiolase; ACAT1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 427

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 4.75pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACAT1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ACAT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ACAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ACAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557237
• NCBI GeneID: 38
• NCBI Accession #: NP_000010.1
• NCBI GenBank Nucleotide #: NM_000019.3
• UniProt Accession #: P24752; Q96FG8; B2R6H1; G3XAB4
• Molecular Weight: 17,175 Da
• NCBI Official Full Name: acetyl-CoA acetyltransferase, mitochondrial
• NCBI Official Synonym Full Names: acetyl-CoA acetyltransferase 1
• NCBI Official Symbol: ACAT1
• NCBI Official Synonym Symbols: T2; MAT; ACAT; THIL
• NCBI Protein Information: acetyl-CoA acetyltransferase, mitochondrial
• UniProt Protein Name: Acetyl-CoA acetyltransferase, mitochondrial
• UniProt Gene Name: ACAT1
• UniProt Synonym Gene Names: ACAT; MAT

NCBI Description

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

Uniprot Description

Plays a major role in ketone body metabolism.