Rabbit anti-Human MRPL28 Polyclonal Antibody | anti-MRPL28 antibody

MRPL28 Antibody

Cat. Num. AAA9403865

• Gene Names: MRPL28; p15; MAAT1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: MRPL28; Polyclonal Antibody; MRPL28 Antibody; p15; MAAT1; anti-MRPL28 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total MRPL28 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 256

• Applicable Applications for anti-MRPL28 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human MRPL28.
• Target Name: MRPL28
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MRPL28 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using MRPL28 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-MRPL28 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.

Product Categories/Family for anti-MRPL28 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 26051229
• NCBI GeneID: 10573
• NCBI Accession #: NP_006419.2
• NCBI GenBank Nucleotide #: NM_006428.4
• UniProt Accession #: Q13084; Q4TT39; Q96S26; Q9BQD8; Q9BR04; B2RCM4; D3DU46
• Molecular Weight: 30,157 Da
• NCBI Official Full Name: 39S ribosomal protein L28, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L28
• NCBI Official Symbol: MRPL28
• NCBI Official Synonym Symbols: p15; MAAT1
• NCBI Protein Information: 39S ribosomal protein L28, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L28, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL28
• UniProt Synonym Gene Names: MAAT1; L28mt; MRP-L28
• UniProt Entry Name: RM28_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL28: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Mitochondrial; Translation

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial ribosome

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: translation; mitochondrial translation; organelle organization and biogenesis