Rabbit anti-Human MRP-S35 Polyclonal Antibody | anti-MRP-S35 antibody

MRP-S35 (I252) Polyclonal Antibody

Cat. Num. AAA3004297

• Gene Names: MRPS35; MDS023; MRPS28; MRP-S28; HDCMD11P
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MRP-S35; Polyclonal Antibody; MRP-S35 (I252) Polyclonal Antibody; 28S ribosomal protein S35; mitochondrial; S35mt; 28S ribosomal protein S28; MRP-S28; S28mt; MRPS35; MRPS28; HDCMD11P; MDS023; PSEC0213; anti-MRP-S35 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MRP-S35 (I252) polyclonal antibody detects endogenous levels of MRP-S35 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 194

• Applicable Applications for anti-MRP-S35 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 220-270 of Human MRP-S35.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MRP-S35 (I252) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:K562 whole cell lysate (40ug))

Related Product Information for anti-MRP-S35 antibody

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4: 1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-S35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRP-S35 is encoded by a gene located on human chromosome 10, which houses over 1, 200 genes and comprises nearly 4.5% of the human genome.

NCBI and Uniprot Product Information

• NCBI GI #: 300068923
• NCBI GeneID: 60488
• NCBI Accession #: NP_001177793.1
• NCBI GenBank Nucleotide #: NP_001177793.1
• UniProt Accession #: P82673
• Molecular Weight: ~ 37kDa
• NCBI Official Full Name: 28S ribosomal protein S35, mitochondrial isoform 2
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S35
• NCBI Official Symbol: MRPS35
• NCBI Official Synonym Symbols: MDS023; MRPS28; MRP-S28; HDCMD11P
• NCBI Protein Information: 28S ribosomal protein S35, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S35, mitochondrial
• UniProt Gene Name: MRPS35
• UniProt Synonym Gene Names: MRPS28; MRP-S35; S35mt; MRP-S28; S28mt
• UniProt Entry Name: RT35_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq, Jul 2010]

Uniprot Description

MRPS35: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: mitochondrion; mitochondrial inner membrane; cytoplasm; mitochondrial small ribosomal subunit

Molecular Function: structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis; DNA damage response, detection of DNA damage