Testing Data
Rabbit anti-Mouse Mre11 Polyclonal Antibody | anti-Mre11 antibody
Anti-Mre11 Antibody
Gene Names
Mre11a; Mre11
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Affinity Purified
Synonyms
Mre11; Polyclonal Antibody; Anti-Mre11 Antibody; anti-Mre11 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This affinity purified antibody is directed against mouse Mre11 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Mre11 protein from rat (94% homology). Based on protein sequence homology also expect partial reactivity against Mre11 homologues from macaque (88%), human (84%) and dog (82%). This sequence shows less than 75% homology to homologues from bovine, Xenopus, chicken, chimpanzee and zebrafish sources. Reactivity against homologues from other sources is not known.
Purity/Purification
Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
706
Applicable Applications for anti-Mre11 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to Mre11 by western blotting in the appropriate cell lysate or extract.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corres-ponding to amino acids 68-706 of mouse Mre11 protein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-Mre11 antibody
Mre11 is a component of the MRN complex (Mre11/Rad50/Nbs1), which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single strand endonuclease activity and double strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Mre11 is a nuclear protein that is widely expressed and localizes to discrete nuclear foci after treatment with genotoxic agents (including irradiation). Alternative splice isoforms are recorded for this protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
MRE11
NCBI Official Synonym Full Names
MRE11 homolog A, double strand break repair nuclease
NCBI Official Symbol
Mre11a
NCBI Official Synonym Symbols
Mre11
NCBI Protein Information
double-strand break repair protein MRE11; double-strand break repair protein MRE11A
Protein Family
NCBI Description
may play a role in regulation of cardiomyocyte proliferation [RGD, Feb 2006]
Similar Products
Product Notes
The Mre11 (Catalog #AAA4157787) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mre11 Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mre11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to Mre11 by western blotting in the appropriate cell lysate or extract. Researchers should empirically determine the suitability of the Mre11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mre11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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