Rabbit MRE11 Polyclonal Antibody | anti-MRE11A antibody

MRE11 Antibody

Cat. Num. AAA9208943

• Gene Names: MRE11A; ATLD; HNGS1; MRE11; MRE11B
• Reactivity: Human, mouse, rat
• Applications: Western Blot, ELISA, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MRE11; Polyclonal Antibody; MRE11 Antibody; Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A; MRE11A; HNGS1; anti-MRE11A antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
• Concentration: 1mg/ml (varies by lot)
• Sequence Positions: 361-420
• Sequence Length: 680

• Applicable Applications for anti-MRE11A antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC)-Paraffin
• Application Notes: WB~~ 1:1000
• Antigen Type: synthetic peptide
• Antigen Source: KLH conjugated synthetic peptide derived from human MRE11
• Preparation and Storage: Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(All lanes : Anti-MRE11 Antibody at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 81 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MRE11A antibody

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

References

Petrini J.H.J.,et al.Genomics 29:80-86(1995).
Petrini J.H.J.,et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
Chamankhah M.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
Paull T.T.,et al.Mol. Cell 1:969-979(1998).
Pitts S.A.,et al.Hum. Mol. Genet. 10:1155-1162(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 24234690
• NCBI GeneID: 4361
• NCBI Accession #: NP_005581.2
• NCBI GenBank Nucleotide #: NM_005590.3
• UniProt Accession #: P49959; O43475; B3KTC7
• Molecular Weight: 80593
• NCBI Official Full Name: double-strand break repair protein MRE11A isoform 2
• NCBI Official Synonym Full Names: MRE11 double strand break repair nuclease A
• NCBI Official Symbol: MRE11A
• NCBI Official Synonym Symbols: ATLD; HNGS1; MRE11; MRE11B
• NCBI Protein Information: double-strand break repair protein MRE11A
• UniProt Protein Name: Double-strand break repair protein MRE11A
• Protein Family: Double-strand break repair protein
• UniProt Gene Name: MRE11A
• UniProt Synonym Gene Names: HNGS1; MRE11; MRE11 homolog 1; MRE11 homolog A
• UniProt Entry Name: MRE11_HUMAN

NCBI Description

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.

Protein type: Deoxyribonuclease; DNA repair, damage; Cell cycle regulation; DNA-binding

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleoplasm; chromosome, telomeric region; Mre11 complex; cytosol; nucleus; chromatin

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; nuclease activity; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; manganese ion binding; endonuclease activity; double-stranded DNA binding; 3'-5' exonuclease activity; endodeoxyribonuclease activity

Biological Process: sister chromatid cohesion; positive regulation of kinase activity; negative regulation of DNA endoreduplication; telomere maintenance via telomerase; regulation of mitotic recombination; DNA repair; positive regulation of protein amino acid autophosphorylation; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA recombination; cell proliferation; base-excision repair; nucleotide-excision repair; intra-S DNA damage checkpoint; meiotic recombination; double-strand break repair; mitotic cell cycle G2/M transition DNA damage checkpoint; synapsis; positive regulation of interferon type I production; innate immune response; telomere maintenance; response to DNA damage stimulus

Disease: Ataxia-telangiectasia-like Disorder 1