Mouse anti-Human MRE11A Monoclonal Antibody | anti-MRE11A antibody

MRE11A (Double-strand Break Repair Protein MRE11A, Meiotic Recombination 11 Homolog 1, MRE11 Homolog 1, Meiotic Recombination 11 Homolog A, MRE11 Homolog A, HNGS1, MRE11) (Biotin)

Cat. Num. AAA6142979

• Gene Names: MRE11A; ATLD; HNGS1; MRE11; MRE11B
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MRE11A; Monoclonal Antibody; MRE11A (Double-strand Break Repair Protein MRE11A; Meiotic Recombination 11 Homolog 1; MRE11 Homolog 1; Meiotic Recombination 11 Homolog A; MRE11 Homolog A; HNGS1; MRE11) (Biotin); anti-MRE11A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1D8-A6
• Specificity: Recognizes human MRE11A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MRE11A antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-206 from human MRE11A (AAH05241) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGRTKIALYGLGSIPDERLYRMFVNKKVTMLRPKED
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (48.4kD).)

Western Blot (WB)

(MRE11A monoclonal antibody Western Blot analysis of MRE11A expression in HL-60.)

Related Product Information for anti-MRE11A antibody

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

Product Categories/Family for anti-MRE11A antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 13528887
• NCBI GeneID: 4361
• Molecular Weight: 81,070 Da
• NCBI Official Full Name: Homo sapiens MRE11 meiotic recombination 11 homolog A (S. cerevisiae), mRNA
• NCBI Official Synonym Full Names: MRE11 homolog A, double strand break repair nuclease
• NCBI Official Symbol: MRE11A
• NCBI Official Synonym Symbols: ATLD; HNGS1; MRE11; MRE11B
• NCBI Protein Information: double-strand break repair protein MRE11A
• Protein Family: Double-strand break repair protein

NCBI Description

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]